UBE2S Human

Ubiquitin Conjugating Enzyme E2S Human Recombinant
Cat. No.
BT19468
Source
Escherichia Coli.
Synonyms
Ubiquitin-conjugating enzyme E2 S, Ubiquitin-protein ligase S, Ubiquitin carrier protein S, Ubiquitin-conjugating enzyme E2-24 kDa, E2-EPF5, E2-EPF, UBE2S, E2EPF, EPF5.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

UBE2S Human Recombinant fused with a 36 amino acids His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 258 amino acids (1-222 a.a.) and having a molecular mass of 27.9kDa.
The UBE2S is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Ubiquitin-conjugating enzyme E2S (UBE2S) is a member of the ubiquitin-conjugating enzyme family. It forms a thiol ester linkage with ubiquitin with the help of a ubiquitin activating enzyme, a characteristic of ubiquitin carrier proteins. UBE2S facilitates the attachment of ubiquitin to other proteins. It plays a key role in the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that regulates mitotic progression. UBE2S specifically elongates 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing their degradation by the proteasome and promoting mitotic exit. Additionally, UBE2S elongates ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro, however, it remains unclear if UBE2D1/UBCH5 functions as an E2 enzyme for APC/C in vivo. UBE2S also participates in the ubiquitination and subsequent degradation of VHL, leading to an increase in HIF1A levels.
Description
Recombinant human UBE2S, with a 36 amino acid His tag at the N-terminus, is produced in E. coli. It is a single, non-glycosylated polypeptide chain comprising 258 amino acids (1-222 a.a.) and has a molecular weight of 27.9kDa. UBE2S is purified using proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The UBE2S solution (1mg/ml) is supplied in a buffer containing 20mM Tris-HCl (pH 8.0), 1mM DTT, and 20% glycerol.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of UBE2S is greater than 90.0% as determined by SDS-PAGE analysis.
Synonyms
Ubiquitin-conjugating enzyme E2 S, Ubiquitin-protein ligase S, Ubiquitin carrier protein S, Ubiquitin-conjugating enzyme E2-24 kDa, E2-EPF5, E2-EPF, UBE2S, E2EPF, EPF5.
Source
Escherichia Coli.
Amino Acid Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMNSN VENLPPHIIR LVYKEVTTLT ADPPDGIKVF PNEEDLTDLQ VTIEGPEGTP YAGGLFRMKL LLGKDFPASP PKGYFLTKIF HPNVGANGEI CVNVLKRDWT AELGIRHVLL TIKCLLIHPN PESALNEEAG RLLLENYEEY AARARLLTEI HGGAGGPSGR AEAGRALASG TEASSTDPGA PGGPGGAEGP MAKKHAGERD KKLAAKKKTD KKRALRRL.

Product Science Overview

Introduction

Ubiquitin Conjugating Enzyme E2S (UBE2S) is a member of the ubiquitin-conjugating enzyme family, also known as E2 enzymes. These enzymes play a crucial role in the ubiquitination process, which is essential for protein degradation and regulation within the cell. UBE2S is particularly significant due to its involvement in various cellular processes, including cell cycle regulation, DNA repair, and signal transduction.

Structure and Function

UBE2S is characterized by its ability to receive ubiquitin from a ubiquitin-activating enzyme (E1) and subsequently interact with a ubiquitin ligase (E3) to conjugate ubiquitin to substrate proteins . This process is vital for targeting proteins for degradation via the proteasome, thus maintaining protein homeostasis within the cell .

Biological Significance

The ubiquitin-proteasome system (UPS) orchestrates the specific poly-ubiquitination of protein substrates in humans, which can lead to their degradation . UBE2S, as part of this system, ensures the proper turnover of proteins, preventing the accumulation of damaged or misfolded proteins that could potentially lead to cellular dysfunction and disease.

Recombinant UBE2S

Recombinant human UBE2S is produced using recombinant DNA technology, which involves inserting the gene encoding UBE2S into a suitable expression system, such as bacteria or yeast. This allows for the large-scale production of UBE2S, which can be used for various research and therapeutic purposes. Recombinant UBE2S retains the functional properties of the native enzyme, making it a valuable tool for studying the ubiquitination process and its implications in health and disease .

Applications in Research

UBE2S is widely used in research to understand its role in the ubiquitination process and its impact on cellular functions. Studies have shown that UBE2S is involved in maintaining proteostasis during aging, ensuring protein quality control, and preventing the accumulation of poly-ubiquitinated proteins . These findings highlight the importance of UBE2S in cellular homeostasis and its potential as a therapeutic target for age-related diseases and other conditions associated with protein misfolding and aggregation.

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