TTC32 Human

Tetratricopeptide Repeat Domain 32 Human Recombinant
Cat. No.
BT11558
Source
E.coli.
Synonyms
Tetratricopeptide Repeat Domain 32, Tetratricopeptide Repeat Protein 32, TPR Repeat Protein 32.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

TTC32 Human Recombinant produced in E. coli is a single polypeptide chain containing 174 amino acids (1-151) and having a molecular mass of 19.7 kDa. TTC32 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
TTC32, a protein containing three tetratricopeptide repeat (TPR) motifs, plays a crucial role in protein-protein interactions. TPR motifs, typically arranged in groups of 3 to 16, create structural scaffolds that facilitate the assembly of multiprotein complexes. Proteins with TPR domains are involved in diverse cellular processes. Examples include cell cycle regulation (e.g., anaphase-promoting complex subunits cdc16, cdc23, and cdc27), immune response (e.g., NADPH oxidase subunit p67-phox and hsp90-binding immunophilins), transcriptional regulation (e.g., transcription factors), protein transport (e.g., peroxisomal import protein PEX5 and mitochondrial import proteins), and protein kinase regulation (e.g., PKR protein kinase inhibitor).
Description
Recombinant human TTC32, expressed in E. coli, is a single polypeptide chain with a molecular weight of 19.7 kDa. This protein comprises 174 amino acids, including the 151 amino acids of TTC32 and a 23 amino acid His-tag fused to the N-terminus. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
TTC32 is supplied as a 0.5 mg/ml solution in 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 2mM DTT, and 20% glycerol.
Stability
For short-term storage (up to 4 weeks), keep at 4°C. For long-term storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
Tetratricopeptide Repeat Domain 32, Tetratricopeptide Repeat Protein 32, TPR Repeat Protein 32.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMEGQRQE SHATLTLAQA HFNNGEYAEA EALYSAYIRR CACAASSDES PGSKCSPEDL ATAYNNRGQI KYFRVDFYEA MDDYTSAIEV QPNFEVPYYN RGLILYRLGY FDDALEDFKK VLDLNPGFQD ATLSLKQTIL DKEEKQRRNV AKNY.

Product Science Overview

Introduction

Tetratricopeptide Repeat Domain 32 (TTC32) is a protein encoded by the TTC32 gene in humans. This protein is part of a larger family of proteins characterized by the presence of tetratricopeptide repeat (TPR) motifs. TPR motifs are structural motifs that consist of a degenerate 34 amino acid tandem repeat, which are found in a wide variety of proteins and play a crucial role in mediating protein-protein interactions .

Structure and Function

The TPR motif is typically composed of a pair of antiparallel alpha helices. These motifs are found in tandem arrays of 3–16 motifs, forming scaffolds that mediate protein-protein interactions and often the assembly of multiprotein complexes . The TPR domain usually folds into a single, linear solenoid structure, which is essential for its function in various cellular processes.

Proteins containing TPR motifs are involved in a wide range of biological functions, including the regulation of the cell cycle, protein folding, and the assembly of protein complexes. For example, TPR motifs are found in the anaphase-promoting complex (APC) subunits, NADPH oxidase subunit p67-phox, hsp90-binding immunophilins, transcription factors, and mitochondrial import proteins .

TTC32 Gene

The TTC32 gene, also known as Tetratricopeptide Repeat Domain 32, is a protein-coding gene. Gene Ontology (GO) annotations related to this gene include identical protein binding . An important paralog of this gene is ST13 . The TTC32 gene is located on chromosome 2 and has several aliases, including TPR Repeat Protein 32 .

Applications and Research

Human recombinant TTC32 is used in various research applications to study protein-protein interactions and the assembly of multiprotein complexes. Understanding the structure and function of TTC32 can provide insights into its role in cellular processes and its potential implications in diseases.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

TTC1 Human
Tetratricopeptide Repeat Domain 1 Human Recombinant
Cat. No.
BT11293
Source
Escherichia Coli.
TTC33 Human
Tetratricopeptide Repeat Domain 33 Human Recombinant
Cat. No.
BT11640
Source
E.coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.