TRIM33 Human

Tripartite Motif Containing 33 Human Recombinant
Cat. No.
BT18824
Source
Sf9 Insect Cells.
Synonyms
E3 ubiquitin-protein ligase TRIM33, Ectodermin homolog, RET-fused gene 7 protein, Protein Rfg7, Transcription intermediary factor 1-gamma, TIF1-gamma, Tripartite motif-containing protein 33, TRIM33, KIAA1113, RFG7, TIF1G, ECTO, PTC7, TF1G, TIFGAMMA, TIF1GAMMA.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TRIM33 Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 124,041 Dalton.
TRIM33 is expressed with a -10xHis tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
TRIM33, also known as Tripartite Motif Containing 33, is a protein that belongs to the tripartite motif family. This family is characterized by the presence of three zinc-binding domains: a RING domain, a B-box type 1, and a B-box type 2, along with a coiled-coil region. TRIM33 is believed to function as a transcriptional corepressor, participating in the regulation of gene expression. It acts as an E3 ubiquitin-protein ligase, facilitating the ubiquitination of target proteins. One such target is SMAD4, a key mediator in the TGF-beta/BMP signaling pathway. TRIM33 promotes SMAD4 ubiquitination, leading to its nuclear exclusion and subsequent degradation via the ubiquitin proteasome pathway. This action effectively inhibits the transcriptional response downstream of TGF-beta/BMP signaling. Additionally, TRIM33 plays a role in controlling cell proliferation.
Description
This product consists of the recombinant human TRIM33 protein, produced in SF9 insect cells. It is a glycosylated polypeptide chain with a calculated molecular weight of 124,041 Daltons. The protein includes a -10xHis tag located at its N-terminus, facilitating purification using proprietary chromatographic techniques.
Physical Appearance
The product appears as a clear solution that has undergone sterile filtration.
Formulation
The provided TRIM33 protein is formulated in a buffer containing 20mM HEPES (pH 7.9), 550mM NaCl, and 6M Urea.
Stability
For short-term storage (2-4 weeks), the product should be kept at a refrigerated temperature of 4°C. For extended storage, it is recommended to freeze the product at -20°C. It is important to avoid repeated cycles of freezing and thawing to maintain product integrity.
Purity
The purity of the TRIM33 protein provided is greater than 80%, as determined by SDS-PAGE analysis.
Synonyms
E3 ubiquitin-protein ligase TRIM33, Ectodermin homolog, RET-fused gene 7 protein, Protein Rfg7, Transcription intermediary factor 1-gamma, TIF1-gamma, Tripartite motif-containing protein 33, TRIM33, KIAA1113, RFG7, TIF1G, ECTO, PTC7, TF1G, TIFGAMMA, TIF1GAMMA.
Source
Sf9 Insect Cells.

Product Science Overview

Gene and Protein Structure

The TRIM33 gene is located on chromosome 1p13.2 in humans . The protein structure includes:

  • RING finger domain: Involved in ubiquitin ligase activity.
  • B-box domains: Contribute to protein-protein interactions.
  • Coiled-coil region: Facilitates oligomerization.

Three alternatively spliced transcript variants of this gene have been described, although the full-length nature of one variant has not been determined .

Biological Functions

TRIM33 functions primarily as a transcriptional corepressor and an E3 ubiquitin-protein ligase . It plays a crucial role in various biological processes, including:

  • Regulation of transcription: TRIM33 negatively regulates transcription by RNA polymerase II and is involved in the regulation of the transforming growth factor-beta (TGF-β) receptor signaling pathway .
  • Protein ubiquitination: It is involved in the ubiquitination and subsequent proteasomal degradation of target proteins .
  • Tumor suppression: TRIM33 acts as a tumor suppressor gene, preventing the development of chronic myelomonocytic leukemia .
Expression Patterns

TRIM33 is expressed in various tissues, including the secondary oocyte, epithelium of the choroid plexus, Brodmann area 23, corpus epididymis, nipple, cerebellar vermis, tibia, and region I of the hippocampus proper . It is also expressed in the genital tubercle, primitive streak, tail of the embryo, ciliary body, zygote, trigeminal ganglion, iris, and retinal pigment epithelium .

Interactions and Pathways

TRIM33 interacts with several proteins, including TRIM24 and SMAD4 . It is involved in multiple signaling pathways, such as the TGF-β receptor signaling pathway and the bone morphogenetic protein (BMP) signaling pathway .

Clinical Significance

TRIM33 has been implicated in various diseases, particularly in cancer. It functions as a tumor suppressor and is involved in the regulation of hematopoietic stem cell aging . Its role in protein ubiquitination and transcriptional regulation makes it a potential target for therapeutic interventions.

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