TRIM21 Human
Greater than 95% as determined by SDS-PAGE.
Description
TRIM21 Human Recombinant produced in SF9 is a polypeptide chain having a calculated molecular mass of 52 kDa.
TRIM21 is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Tripartite Motif Containing 21 (TRIM21), also known as RO52, is a protein encoded by the TRIM21 gene in humans. This protein is a member of the tripartite motif (TRIM) family, which is characterized by the presence of three zinc-binding domains: a RING finger domain, a B-box type 1, and a B-box type 2, along with a coiled-coil region . TRIM21 plays a crucial role in the immune system and is involved in various cellular processes, including protein ubiquitination and intracellular antibody-mediated proteolysis .
TRIM21 is expressed in most human tissues and is particularly abundant in immune cells . It functions as an E3 ubiquitin-protein ligase, which means it helps attach ubiquitin molecules to specific protein substrates, marking them for degradation by the proteasome . This activity is essential for maintaining cellular homeostasis and regulating immune responses.
One of the unique functions of TRIM21 is its role in intracellular immunity. It acts as a sensor that intercepts antibody-coated viruses that have evaded extracellular neutralization and breached the cell membrane . By binding to the Fc domain of immunoglobulins, TRIM21 can target these viruses for degradation, providing a last line of defense against viral infections .
TRIM21 is closely associated with autoimmune diseases, particularly systemic lupus erythematosus (SLE) and primary Sjögren’s syndrome (pSS) . Dysregulation of TRIM21 can lead to aberrant immune responses, contributing to the pathogenesis of these conditions. Autoantibodies against TRIM21 are commonly found in patients with these autoimmune diseases, making it a valuable biomarker for diagnosis and disease monitoring .
Recombinant TRIM21 is a human-derived protein that is expressed in Escherichia coli (E. coli) for research and therapeutic purposes . This recombinant protein retains the functional properties of the native TRIM21 and is used in various studies to understand its role in immune regulation and disease mechanisms. Additionally, recombinant TRIM21 has been shown to interact with the HIV-1 Vpu protein, highlighting its potential in antiviral research .
