TRAPPC4 Human

Trafficking Protein Particle Complex 4 Human Recombinant
Cat. No.
BT14728
Source
Escherichia Coli.
Synonyms
Trafficking protein particle complex subunit 4, TRS23 homolog, Synbindin, Hematopoietic stem/progenitor cell protein 172.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TRAPPC4 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 242 amino acids (1-219) and having a molecular mass of 26.7kDa. 
TRAPPC4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Trafficking protein particle complex 4 (TRAPPC4), a component of the multisubunit TRAPP (transport protein particle) complex, interacts with SDC2 and plays a role in vesicular transport from the endoplasmic reticulum to the Golgi apparatus.
Description
Recombinant human TRAPPC4, expressed in E. coli, is a non-glycosylated polypeptide chain containing 242 amino acids (residues 1-219) with a molecular weight of 26.7 kDa. It includes a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile filtered solution.
Formulation
The TRAPPC4 protein solution (0.25 mg/ml) is supplied in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.2 M NaCl, 20% glycerol, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the protein at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
The purity is determined to be greater than 90% by SDS-PAGE analysis.
Synonyms
Trafficking protein particle complex subunit 4, TRS23 homolog, Synbindin, Hematopoietic stem/progenitor cell protein 172.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAIFSVY VVNKAGGLIY QLDSYAPRAE AEKTFSYPLD LLLKLHDERV LVAFGQRDGI RVGHAVLAIN GMDVNGRYTA DGKEVLEYLG NPANYPVSIR FGRPRLTSNE KLMLASMFHS LFAIGSQLSP EQGSSGIEML ETDTFKLHCY QTLTGIKFVV LADPRQAGID SLLRKIYEIY SDFALKNPFY SLEMPIRCEL FDQNLKLALE VAEKAGTFGP GS.

Product Science Overview

Introduction

Trafficking Protein Particle Complex 4 (TRAPPC4) is a crucial component of the TRAPP (Transport Protein Particle) complex, which plays a significant role in intracellular vesicular trafficking. This complex is involved in various cellular processes, including secretion, autophagy, and vesicle-mediated transport from the endoplasmic reticulum to the Golgi apparatus .

Structure and Function

TRAPPC4 is a highly conserved protein that acts as a guanine nucleotide exchange factor (GEF) for Rab1 GTPase . This activity is essential for the regulation of vesicular trafficking events. The protein is also known to interact with other components of the TRAPP complex, facilitating the formation and function of the complex .

Biological Significance

The TRAPP complex, including TRAPPC4, is vital for maintaining cellular homeostasis. It ensures the proper transport of proteins and lipids within the cell, which is crucial for various cellular functions. Disruptions in the function of TRAPPC4 can lead to severe cellular dysfunctions and are associated with several neurodevelopmental disorders .

Recombinant Human TRAPPC4

Recombinant human TRAPPC4 is produced using recombinant DNA technology, where the TRAPPC4 gene is cloned and expressed in a suitable host system, such as E. coli. The recombinant protein is then purified using conventional chromatography techniques . This recombinant form is used in various research applications to study the protein’s function and its role in cellular processes.

Applications in Research

Recombinant human TRAPPC4 is widely used in biochemical and cellular studies to understand its role in vesicular trafficking and autophagy. It is also used to investigate the molecular mechanisms underlying neurodevelopmental disorders associated with TRAPPC4 dysfunction .

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THE BIOTEK
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