TPSAB1 Human, Sf9

Tryptase Alpha/Beta 1, also known as TPSAB1, is a tryptase enzyme that is a key neutral protease present in mast cells. It is enzymatically active only as a heparin-stabilized tetramer and is resistant to all known endogenous proteinase inhibitors .

Tryptase Alpha/Beta 1 is a trypsin-like serine protease. Beta tryptases are the main isoenzymes expressed in mast cells. These enzymes are stored in the secretory granules of mast cells, where they form active tetramers with heparin proteoglycan . The unique arrangement of the active sites in the tetramer, which face a narrow central pore, makes beta tryptases resistant to macromolecule protease inhibitors .

When mast cells are activated, beta tryptases are released along with other proteins in secretory granules. They participate in provoking inflammatory conditions and have been implicated as mediators in the pathogenesis of asthma and other allergic disorders . Tryptase Alpha/Beta 1 plays a crucial role in the coupled activation-degranulation response of mast cells .

Tryptase Alpha/Beta 1 is enzymatically active only as a heparin-stabilized tetramer. This tetrameric form is resistant to all known endogenous proteinase inhibitors, which allows it to maintain its activity in various physiological conditions . The enzyme cleaves specific peptide substrates, contributing to its role in inflammatory responses .

The activity of Tryptase Alpha/Beta 1 is regulated by its interaction with heparin proteoglycan, which stabilizes its tetrameric form. This interaction is crucial for its enzymatic activity and resistance to inhibitors . Additionally, the release of beta tryptases from mast cells is tightly regulated and occurs in response to specific stimuli that trigger mast cell activation .

The recombinant form of Tryptase Alpha/Beta 1 (Human Recombinant, Sf9) is produced using the Sf9 insect cell expression system. This system allows for the production of high-quality recombinant proteins that retain their biological activity and structural integrity . The recombinant protein is purified using proprietary chromatographic techniques to ensure its purity and functionality .

Recombinant Tryptase Alpha/Beta 1 is used in various research applications, including studies on mast cell biology, inflammatory responses, and allergic disorders. It is also utilized in drug development and screening assays to identify potential inhibitors of tryptase activity .