TPSAB1 Human
Description
TPSAB1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Tryptase alpha/beta-1 is a trypsin-like serine protease. The enzyme is stored in the secretory granules of mast cells in an inactive form and is released upon the activation and degranulation of these cells . When released, it forms active tetramers stabilized by heparin proteoglycan . This unique tetrameric structure makes the enzyme resistant to all known endogenous proteinase inhibitors .
The enzyme’s active sites are arranged in such a way that they face a narrow central pore, which contributes to its resistance to inhibition . This structural arrangement is essential for its function in the extracellular matrix, where it participates in various physiological and pathological processes.
Tryptase alpha/beta-1 is involved in several critical biological processes, including:
- Inflammation: It plays a significant role in the inflammatory response by breaking down extracellular matrix components and activating other proteases .
- Allergic Reactions: The enzyme is a mediator in the pathogenesis of asthma and other allergic disorders. It contributes to the symptoms of these conditions by promoting the release of histamine and other inflammatory mediators from mast cells .
- Tissue Remodeling: Tryptase alpha/beta-1 is involved in tissue remodeling and repair by degrading extracellular matrix proteins and facilitating cell migration .
Recombinant human tryptase alpha/beta-1 is produced using advanced biotechnological methods. The enzyme is typically expressed in a mouse myeloma cell line (NS0) and purified to high levels of purity (>95%) using techniques such as SDS-PAGE under reducing conditions . The recombinant form is often tagged with a C-terminal 10-His tag to facilitate purification and detection .
The recombinant enzyme is supplied as a carrier-free formulation, which means it does not contain bovine serum albumin (BSA). This formulation is particularly useful for applications where the presence of BSA could interfere with experimental results .
