TPM1 Human

Tropomyosin 1 (TPM1) belongs to the tropomyosin protein family, known for their highly conserved nature and widespread distribution. These proteins, with molecular weights ranging from 35 to 45 kDa, play crucial roles in the contractile systems of both striated and smooth muscles, as well as in the cytoskeleton of non-muscle cells. TPM1 itself is a dimer composed of two alpha-helical chains forming a coiled-coil structure. This protein polymerizes along the actin filaments, specifically within the grooves, contributing to filament stability. Its interaction with actin is observed in both muscle and non-muscle cells. In muscle cells, TPM1 collaborates with the troponin complex to regulate the calcium-dependent interaction between actin and myosin, essential for muscle contraction. Conversely, in non-muscle cells, TPM1 participates in stabilizing cytoskeleton actin filaments. Notably, in smooth muscle cells, the interaction between TPM1 and caldesmon governs smooth muscle contraction. Several alternatively spliced transcript variants of TPM1 exist, encoding a variety of isoforms with distinct expression patterns. For instance, Isoform 1 is found in both adult and fetal skeletal and cardiac muscles, exhibiting higher expression in cardiac tissues. On the other hand, Isoform 10 is specifically expressed in adult and fetal cardiac tissues, absent in skeletal muscle. Mutations in the TPM1 gene are implicated in the development of type 3 familial hypertrophic cardiomyopathy.

Recombinant human TPM1, expressed in E. coli, is a single, non-glycosylated polypeptide chain. This protein consists of 304 amino acids, with a sequence spanning from amino acid positions 1 to 284, resulting in a molecular mass of 35 kDa. However, it's important to note that the apparent molecular weight on SDS-PAGE may be higher. The recombinant TPM1 protein is engineered with a 20 amino acid His Tag fused to its N-terminus. Purification is achieved through proprietary chromatographic methods.

The product is a clear solution that has been sterilized by filtration.

The TPM1 protein solution has a concentration of 0.5 mg/ml and is supplied in a buffer consisting of 20 mM Tris-HCl (pH 8), 1 mM DTT, 0.1 M NaCl, and 20% glycerol.

For short-term storage (up to 4 weeks), the unopened vial should be kept refrigerated at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure optimal stability during long-term storage, the addition of a carrier protein such as HSA or BSA (0.1%) is advisable. Repeated freeze-thaw cycles should be minimized.

The purity of the TPM1 protein is determined by SDS-PAGE analysis and is consistently greater than 90.0%.

Tropomyosin alpha-1 chain, Tropomyosin-1, Alpha-tropomyosin, TPM1, C15orf13, TMSA, CMD1Y, HTM-alpha.

Escherichia Coli.

MGSSHHHHHH SSGLVPRGSH MDAIKKKMQM LKLDKENALD RAEQAEADKK AAEDRSKQLE DELVSLQKKL KGTEDELDKY SEALKDAQEK
LELAEKKATD AEADVASLNR RIQLVEEELD RAQERLATAL QKLEEAEKAA DESERGMKVI ESRAQKDEEK MEIQEIQLKE AKHIAEDADR
KYEEVARKLV IIESDLERAE ERAELSEGQV RQLEEQLRIM DQTLKALMAA EDKYSQKEDR YEEEIKVLSD KLKEAETRAE FAERSVTKLE
KSIDDLEDEL YAQKLKYKAI SEELDHALND MTSM.