TPD52L1 Antibody
Description
Product Specs
Product Science Overview
Tumor Protein D52 L1 (TPD52L1) is a member of the Tumor Protein D52 (TPD52) family, which is known for its role in cellular proliferation, survival, and vesicle trafficking. The TPD52 family consists of several proteins, including TPD52, TPD52L1, TPD52L2, and TPD52L3, which share a conserved coiled-coil domain that facilitates protein-protein interactions .
TPD52L1 is expressed in various tissues and has been implicated in several types of cancer, including breast, prostate, and ovarian cancers. The overexpression of TPD52L1 has been associated with tumorigenesis, suggesting that it plays a critical role in cancer development and progression . Studies have shown that TPD52L1 can interact with other proteins involved in cell cycle regulation and apoptosis, further highlighting its importance in cancer biology .
Mouse Anti-Human TPD52L1 antibodies are monoclonal antibodies generated by immunizing mice with human TPD52L1 protein. These antibodies are highly specific and can be used in various applications, including Western blotting, immunohistochemistry, and flow cytometry . The primary purpose of these antibodies is to detect and quantify TPD52L1 expression in human tissues and cell lines, aiding in cancer research and diagnostics .
The production of Mouse Anti-Human TPD52L1 antibodies involves several steps:
- Immunization: Mice are immunized with purified human TPD52L1 protein to elicit an immune response.
- Hybridoma Production: Spleen cells from the immunized mice are fused with myeloma cells to create hybridoma cells, which can produce monoclonal antibodies.
- Screening and Selection: Hybridoma cells are screened for the production of antibodies specific to TPD52L1. Positive clones are selected and expanded.
- Purification: Monoclonal antibodies are purified from the culture supernatant using protein A/G affinity chromatography.
These antibodies are stored at -20°C to maintain their stability and activity .
The use of Mouse Anti-Human TPD52L1 antibodies has several important applications in cancer research:
- Detection and Quantification: These antibodies can be used to detect and quantify TPD52L1 expression in various cancer cell lines and tissues, providing valuable information about the role of TPD52L1 in cancer development and progression .
- Therapeutic Targeting: Understanding the expression and function of TPD52L1 in cancer can help identify potential therapeutic targets. Inhibiting TPD52L1 expression or function may offer a novel approach to cancer treatment .
- Diagnostic Marker: TPD52L1 expression levels can serve as a diagnostic marker for certain types of cancer, aiding in early detection and diagnosis .
