T.pallidum p41
Treponema pallidum p41 Recombinant
Cat. No.
BT8737
Source
Escherichia Coli.
Synonyms
Appearance
Purity
Treponema pallidum protein is >90% pure as determined by 10% PAGE (coomassie staining).
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
The E.coli derived recombinant protein contains the outer membrane T.Pallidum p41 immunodominant regions. The protein is fused with a Beta-galactosidase tag.
Product Specs
Introduction
Treponema pallidum, a gram-negative spirochaete bacterium, exhibits limited metabolic capabilities due to its adaptation to the nutrient-rich environment of mammalian tissue. This bacterium, known to have at least four subspecies (T. pallidum pallidum, T. pallidum pertenue, T. pallidum carateum, and T. pallidum endemicum), possesses a helical structure enabling corkscrew motion through viscous substances like mucus. Notably, T. pallidum pallidum has a remarkably small genome (1.14 million base pairs) indicative of its reduced metabolic capacity.
Description
This recombinant protein, derived from E. coli, encompasses the immunodominant regions of the outer membrane T.Pallidum p41 protein. It is engineered with a Beta-galactosidase tag for various applications.
Purity
The purity of the Treponema pallidum protein exceeds 90%, as assessed by 10% PAGE (Coomassie staining).
Formulation
The protein is supplied in a buffer containing 10mM Tris-HCl (pH 8.0), 1mM EDTA, 1mM DTT, and 8M urea.
Stability
For optimal stability, T.pallidum p41 should be stored below -18°C. While it can remain stable at 4°C for up to one week, repeated freeze-thaw cycles should be avoided.
Applications
Treponema pallidum serves as a valuable tool for ELISA and Western blot analyses. Its exceptional antigenic properties make it ideal for the specific detection of T. Pallidum, minimizing cross-reactivity issues.
Source
Escherichia Coli.
Purification Method
Treponema pallidum protein was purified by proprietary chromatographic technique.
Specificity
Immunoreactive with sera of T.Pallidum infected individuals.
Product Science Overview
Structure and Function of p41 Protein
The p41 protein, also known as flagellin, is a major component of the flagella in Treponema pallidum. The flagella are crucial for the bacterium’s motility, enabling it to move through viscous environments such as mucus and tissue. The p41 protein is immunodominant, meaning it elicits a strong immune response in infected individuals .
Recombinant p41 Protein
Recombinant p41 protein is produced using genetic engineering techniques. The gene encoding the p41 protein is cloned and expressed in a host organism, typically Escherichia coli (E. coli). This allows for the production of large quantities of the protein, which can be purified and used for various applications .
Applications of Recombinant p41 Protein
- Diagnostic Testing: Recombinant p41 protein is used in serological tests to diagnose syphilis. The protein’s immunodominant nature makes it an excellent antigen for detecting antibodies in the blood of infected individuals .
- Research: The recombinant protein is used in research to study the structure and function of Treponema pallidum’s flagella, as well as the bacterium’s mechanisms of motility and immune evasion.
- Vaccine Development: Although there is currently no vaccine for syphilis, recombinant proteins like p41 are being investigated as potential components of a future vaccine.
Production and Purification
The production of recombinant p41 protein involves several steps:
- Cloning: The gene encoding the p41 protein is inserted into a plasmid vector, which is then introduced into E. coli cells.
- Expression: The E. coli cells are cultured under conditions that induce the expression of the p41 protein.
- Purification: The protein is purified from the bacterial cells using techniques such as affinity chromatography. The purified protein is typically fused to a tag (e.g., 6 His TAG) to facilitate purification and detection .
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
