T.pallidum p15 (Partial), His

Treponema pallidum is a spirochete bacterium responsible for syphilis, a chronic and complex sexually transmitted disease. The bacterium has a small genome of approximately 1.14 million base pairs, reflecting its adaptation to the rich environment of mammalian tissue . The p15 protein of Treponema pallidum is a significant immunogen during natural syphilis infection in humans and experimental infection in other hosts .

The p15 protein is a 15 kDa lipoprotein that plays a crucial role in the immune response to Treponema pallidum. It is a major membrane immunogen, meaning it is a key target for the immune system during infection . The humoral and cellular immune responses to this molecule appear late in infection as resistance to reinfection is developing .

The recombinant p15 (partial) protein is produced using Escherichia coli (E. coli) expression systems. This recombinant protein includes the immunodominant regions of the p15 protein and is fused with a His tag at the C-terminus . The His tag facilitates purification and detection of the recombinant protein.

The recombinant p15 (partial) protein is used in various applications, including:

• Antigen in ELISA and Western blots: It serves as an excellent antigen for detecting Treponema pallidum with minimal specificity problems .
• Research: It is used in studies related to the immune response to Treponema pallidum and the development of diagnostic tools for syphilis .

The recombinant p15 (partial) protein is typically supplied in a liquid form containing 70 mM Tris-HCl pH 8.0, 50 mM NaCl, 50% glycerol, and 1.5 M urea . It should be stored at -20°C to maintain its stability and avoid freeze/thaw cycles .