TPA (311-562) Human
Tissue Plasminogen Activator (311-562 a.a.) Human Recombinant
Cat. No.
BT28841
Source
Sf9, Baculovirus cells.
Synonyms
Tissue-type plasminogen activator, EC 3.4.21.68, tPA, t-PA, t-plasminogen activator, TPA, T-PA, DKFZp686I03148, PLAT and tPA.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
TPA Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 261 amino acids (311-562 a.a.) and having a molecular mass of 29.2kDa (Migrates at 28-40kDa on SDS-PAGE under reducing conditions).
TPA is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
TPA is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
Tissue plasminogen activator (tPA or PLAT) is a serine protease that activates plasminogen into plasmin, a fibrinolytic enzyme responsible for breaking down blood clots. tPA is naturally occurring and plays a crucial role in dissolving blood clots, thereby aiding in the treatment of conditions such as stroke and heart attack. This enzyme is also involved in cell migration and tissue remodeling.
Description
This product consists of the recombinant form of human tissue plasminogen activator (tPA), specifically amino acids 311 to 562. It is produced using Sf9 insect cells and undergoes glycosylation, a process crucial for its biological activity. The protein, with a molecular weight of 29.2 kDa, is engineered to carry a 6-amino acid His tag at its C-terminus to facilitate purification. The final product is highly pure, exceeding 95% purity, as confirmed by SDS-PAGE analysis.
Physical Appearance
Clear, colorless solution, devoid of any particulate matter.
Formulation
The tPA protein is provided in a solution containing 50mM MES (pH 5.0), 5mM CaCl2, 1mM DTT, 0.5M NaCl, and 30% glycerol. This specific formulation helps maintain the stability and activity of the protein.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the product at -20°C. To further enhance stability during long-term storage, the addition of a carrier protein such as HSA or BSA (0.1%) is advised. Repeated freezing and thawing should be avoided to maintain the integrity of the protein.
Purity
High purity exceeding 95%, determined by SDS-PAGE.
Synonyms
Tissue-type plasminogen activator, EC 3.4.21.68, tPA, t-PA, t-plasminogen activator, TPA, T-PA, DKFZp686I03148, PLAT and tPA.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
ADPIKGGLFA DIASHPWQAA IFAKHRRSPG ERFLCGGILI SSCWILSAAH CFQERFPPHH LTVILGRTYR VVPGEEEQKF EVEKYIVHKE FDDDTYDNDI ALLQLKSDSS RCAQESSVVR TVCLPPADLQ LPDWTECELS GYGKHEALSP FYSERLKEAH VRLYPSSRCT SQHLLNRTVT DNMLCAGDTR SGGPQANLHD ACQGDSGGPL VCLNDGRMTL VGIISWGLGC GQKDVPGVYT KVTNYLDWIR DNMRPHHHHH H.
Product Science Overview
Introduction
Tissue Plasminogen Activator (tPA) is a serine protease enzyme that plays a crucial role in the breakdown of blood clots. It is primarily used in the treatment of ischemic stroke and other thrombotic conditions. The recombinant form of tPA, particularly the segment spanning amino acids 311-562, has been extensively studied and utilized in various therapeutic applications.
Structure and Function
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