TOP1 Human
Description
DNA Topoisomerase-I Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 102 kDa. The TOP1 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
TOP1 is a nuclear enzyme that plays a vital role in maintaining the supercoiling and torsional tension of DNA. It achieves this by transiently cleaving and rejoining one strand of the DNA duplex. The enzyme introduces a single-strand break via a transesterification reaction at a target site in the DNA. This allows the free DNA strand to rotate around the intact phosphodiester bond on the opposing strand, thereby removing DNA supercoils .
The enzyme’s catalytic activity involves the formation of a DNA-(3’-phosphotyrosyl)-enzyme intermediate, which is crucial for the relaxation of supercoiled DNA molecules . This process is essential for normal DNA replication and transcription, as it prevents the formation of knots and tangles in the DNA that could otherwise impede these processes .
TOP1 is essential for normal cellular development and transcriptional regulation. It is involved in the circadian transcription of the core circadian clock component BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the BMAL1 promoter . Additionally, TOP1 regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells .
Variants in the TOP1 gene have been associated with various diseases, including hematologic cancers and spinocerebellar ataxia . The enzyme is also a target antigen for Scl-70 autoantibodies, which are associated with systemic sclerosis .
Recombinant DNA Topoisomerase-I is produced using recombinant DNA technology, which involves inserting the human TOP1 gene into a suitable expression system, such as bacteria or yeast. This allows for the large-scale production of the enzyme for research and therapeutic purposes .
Recombinant TOP1 is used in various biochemical assays to study its function and interactions with other molecules. It is also employed in drug discovery efforts aimed at identifying inhibitors of TOP1, which could be potential therapeutic agents for treating cancers and viral infections .
TOP1 inhibitors, such as camptothecin and its derivatives, are used as chemotherapeutic agents due to their ability to stabilize the TOP1-DNA complex, leading to DNA damage and cell death in rapidly dividing cancer cells . Additionally, research has shown that TOP1 can repress HIV-1 promoter activity through its interaction with a guanine quadruplex present in the LTR sequence, offering new perspectives for anti-viral strategies .
