TNFSF14 Human
Escherichia Coli.
Tumor necrosis factor ligand superfamily member 14, CD258, Tnfsf14, Light
Sterile Filtered clear solution.
Protein is >95% pure as determined by 10% PAGE (coomassie staining).
Description
Recombinant Human LIGHT (74-240 aa) having a Mw of 23kDa was purified from E. coli.
The Recombinant Human LIGHT is purified by proprietary chromatographic technique.
Product Specs
Tumor necrosis factor ligand superfamily member 14, CD258, Tnfsf14, Light
Escherichia Coli.
DGPAGSWEQL IQERRSHEVN PAAHLTGANS SLTGSGGPLL WETQLGLAFL RGLSYHDGAL VVTKAGYYYI YSKVQLGGVG CPLGLASTIT HGLYKRTPRY PEELELLVSQ QSPCGRATSS SRVWWDSSFL GGVVHLEAGE KVVVRVLDER LVRLRDGTRS YFGAFMV
Product Science Overview
LIGHT is a type II transmembrane protein, meaning it spans the membrane with its N-terminus inside the cell and its C-terminus outside . It is expressed by activated T lymphocytes, monocytes, granulocytes, spleen cells, and immature dendritic cells . The protein is composed of 240 amino acids and has a molecular mass of approximately 20.9 kDa .
LIGHT interacts with several receptors, including HVEM (Herpesvirus entry mediator) and LTβR (Lymphotoxin-beta receptor), to mediate its effects . These interactions are critical for the regulation of immune responses, including the activation of T cells and the induction of apoptosis in certain cell types . LIGHT is also involved in the development of secondary lymphoid organs and the maintenance of immune homeostasis .
Recombinant human LIGHT is produced using various expression systems, including mouse myeloma cell lines . The recombinant protein is often used in research to study its role in immune responses and its potential therapeutic applications . It is typically purified to a high degree of purity (>95%) and tested for endotoxin levels to ensure its suitability for experimental use .
Recombinant LIGHT has several applications in biomedical research. It is used to study:
- Immune Regulation: Understanding how LIGHT modulates immune responses can provide insights into autoimmune diseases and potential therapeutic targets.
- Cancer Research: LIGHT’s ability to induce apoptosis in certain cell types makes it a candidate for cancer therapy research.
- Vaccine Development: LIGHT’s role in activating T cells can be leveraged to enhance vaccine efficacy.
