TNFRSF4 Human
TNFRSF4, TNF Receptor Superfamily Member 4, TAX Transcriptionally-Activated Glycoprotein 1 Receptor, OX40L Receptor, ACT35 Antigen, CD134 Antigen, TXGP1L, Tax-Transcriptionally Activated Glycoprotein 1 Receptor, Tumor Necrosis Factor Receptor Superfamily, Member 4, Tumor Necrosis Factor Receptor Superfamily Member 4 , Lymphoid Activation Antigene ACT35, OX40 Cell Surface Antigen, OX40 Homologue , ATC35 Antigen, OX40 Antigen , ACT35, CD134, IMD16, OX40.
Greater than 90.0% as determined by SDS-PAGE.
Description
TNFRSF4 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 425 amino acids (29-214a.a.) and having a molecular mass of 46.9Da. (Molecular size on SDS-PAGE will appear at approximately 40-57kDa). TNFRSF4 is expressed with a 239 amino acid higG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
TNFRSF4, also known as TNF Receptor Superfamily Member 4, is a co-stimulatory molecule found on T cells and belongs to the TNF receptor superfamily. It plays a crucial role in the immune response by working with other co-stimulatory molecules like CD28, CD40, CD30, CD27, and 4-1BB. TNFRSF4 is essential for the expansion and survival of antigen-specific T cells. Its expression increases on CD4+ and CD8+ T cells when the T cell receptor (TCR) interacts with antigens presented by antigen-presenting cells, along with co-stimulation signals from CD40-CD40 Ligand and CD28-B7. Moreover, TNFRSF4 influences the production of cytokines from various immune cells, including T cells, antigen-presenting cells, and natural killer (NK) cells. It also regulates cytokine receptor signaling.
Produced in Sf9 insect cells, TNFRSF4 is a single, glycosylated polypeptide chain with a molecular weight of 46.9 kDa (although it may appear between 40-57 kDa on SDS-PAGE). This recombinant protein encompasses amino acids 29-214 and includes a 239 amino acid IgG-His tag at the C-terminus. Purification is achieved using proprietary chromatographic techniques.
The TNFRSF4 protein solution is provided at a concentration of 0.5 mg/ml in a buffer consisting of Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
The purity of TNFRSF4 is determined to be greater than 90% based on SDS-PAGE analysis.
The biological activity of TNFRSF4 is evaluated through its ability to bind to mouse OX40 Ligand/TNFSF4 in a functional ELISA. The ED50 value, which represents the concentration at which 50% of the maximum binding is observed, is determined to be less than or equal to 0.15 ug/ml.
TNFRSF4, TNF Receptor Superfamily Member 4, TAX Transcriptionally-Activated Glycoprotein 1 Receptor, OX40L Receptor, ACT35 Antigen, CD134 Antigen, TXGP1L, Tax-Transcriptionally Activated Glycoprotein 1 Receptor, Tumor Necrosis Factor Receptor Superfamily, Member 4, Tumor Necrosis Factor Receptor Superfamily Member 4 , Lymphoid Activation Antigene ACT35, OX40 Cell Surface Antigen, OX40 Homologue , ATC35 Antigen, OX40 Antigen , ACT35, CD134, IMD16, OX40.
LHCVGDTYPS NDRCCHECRP GNGMVSRCSR SQNTVCRPCG PGFYNDVVSS KPCKPCTWCN LRSGSERKQL CTATQDTVCR CRAGTQPLDS YKPGVDCAPC PPGHFSPGDN QACKPWTNCT LAGKHTLQPA SNSSDAICED RDPPATQPQE TQGPPARPIT VQPTEAWPRT SQGPSTRPVE VPGGRALEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGKH HHHHH
Product Science Overview
TNFRSF4 is a type I transmembrane protein consisting of 277 amino acids . It is primarily expressed on the surface of activated T cells, including both CD4+ and CD8+ T cells . The protein has an extracellular domain that interacts with its ligand, OX40L (TNFSF4), a transmembrane glycoprotein belonging to the TNF superfamily . The interaction between OX40 and OX40L is essential for the co-stimulation of T cells, which is necessary for their activation and survival .
The primary function of TNFRSF4 is to act as a co-stimulatory molecule in the immune response. When T cells are activated by antigen-presenting cells, TNFRSF4 provides additional signals that enhance T cell proliferation, cytokine production, and survival . This co-stimulatory signal is crucial for the development of long-term T cell immunity, which is essential for the body’s defense against infections and cancer .
Additionally, TNFRSF4 has been implicated in the regulation of various immune responses, including the positive regulation of B cell proliferation and immunoglobulin production . It also plays a role in the inflammatory response and the negative regulation of DNA-binding transcription factor activity .
Due to its role in the immune system, TNFRSF4 has been studied extensively for its potential therapeutic applications. It has been identified as a target for immunotherapy in cancer treatment, as enhancing the OX40-OX40L interaction can boost the immune response against tumors . Furthermore, TNFRSF4 is involved in autoimmune diseases, where its dysregulation can lead to excessive immune activation and tissue damage .
Recombinant TNFRSF4 is produced using genetic engineering techniques to create a synthetic version of the protein. This recombinant protein is used in research to study its function and potential therapeutic applications. It is typically expressed in insect cells and purified to high levels of purity for experimental use .
