TNFRSF4 Mouse
HEK293 cells.
Tumor necrosis factor receptor superfamily member 4, OX40 antigen, OX40L receptor, Txgp1, Tnfrsf4, tax-transcriptionally activated glycoprotein 1 receptor, TXGP1L, ACT35, Txgp, Ly-70, ACT3, OX4, CD134.
Greater than 95.0% as determined by SDS-PAGE.
Description
TNFRSF4 Mouse Recombinant produced in HEK293 cells is a single, glycosylated polypeptide chain (a.a 20-211) containing 435 amino acids and having a molecular mass of 48.6kDa.
TNFRSF4 is fused to a 243 amino acid hIgG-His tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
TNFRSF4, also known as TNF Receptor Superfamily Member 4, is a co-stimulatory molecule found on T cells. It belongs to the TNF receptor superfamily. TNFRSF4 works in concert with other co-stimulatory molecules like CD28, CD40, CD30, CD27, and 4-1BB to regulate immune responses. This receptor plays a crucial role in the expansion and survival of antigen-specific T cells. When the T cell receptor (TCR) encounters antigens presented by antigen-presenting cells, and with the help of co-stimulation from CD40-CD40 Ligand and CD28-B7 interactions, TNFRSF4 expression increases on CD4+ and CD8+ T cells. Furthermore, TNFRSF4 influences the production of cytokines from various immune cells, including T cells, antigen-presenting cells, and natural killer cells. It also plays a role in modulating cytokine receptor signaling.
Recombinant TNFRSF4 from mice, produced in HEK293 cells, is a single-chain polypeptide with glycosylation. It encompasses amino acids 20 to 211, resulting in a protein of 435 amino acids with a molecular weight of 48.6 kDa. The TNFRSF4 protein is fused with a 243 amino acid hIgG-His tag at its C-terminus. Purification is achieved through proprietary chromatographic methods.
The TNFRSF4 protein solution has a concentration of 0.25 mg/ml and is prepared in Phosphate-Buffered Saline (pH 7.4) with 10% glycerol.
Repeated freezing and thawing should be avoided.
The purity is determined to be greater than 95.0% using SDS-PAGE analysis.
The ED50, measured as its binding affinity to Mouse OX40 Ligand/TNFSF4 in a functional ELISA, is less than or equal to 0.4 ug/ml.
Tumor necrosis factor receptor superfamily member 4, OX40 antigen, OX40L receptor, Txgp1, Tnfrsf4, tax-transcriptionally activated glycoprotein 1 receptor, TXGP1L, ACT35, Txgp, Ly-70, ACT3, OX4, CD134.
HEK293 cells.
DGSMVTARRL NCVKHTYPSG HKCCRECQPG HGMVSRCDHT RDTLCHPCET GFYNEAVNYD TCKQCTQCNH RSGSELKQNC TPTQDTVCRC RPGTQPRQDS GYKLGVDCVP CPPGHFSPGN NQACKPWTNC TLSGKQTRHP ASDSLDAVCE DRSLLATLLW ETQRPTFRPT TVQSTTVWPR TSELPSPPTL VTPEGPLEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGKH HHHHH
Product Science Overview
TNFRSF4 is a type I transmembrane protein that consists of an extracellular domain, a transmembrane domain, and a cytoplasmic domain. The extracellular domain is responsible for ligand binding, while the cytoplasmic domain is involved in signal transduction. The primary ligand for TNFRSF4 is OX40L (OX40 Ligand), which is also a member of the TNF superfamily .
The interaction between TNFRSF4 and OX40L is essential for the activation and survival of T cells. This interaction promotes the proliferation and differentiation of T cells, enhances the production of cytokines, and supports the formation of memory T cells. Additionally, TNFRSF4 signaling has been shown to inhibit apoptosis by upregulating anti-apoptotic proteins such as BCL2 and BCL2L1 .
TNFRSF4 is predominantly expressed on activated T cells, particularly CD4+ T cells. It plays a critical role in the regulation of T cell-mediated immune responses. The activation of TNFRSF4 signaling enhances the survival and function of effector T cells, which are responsible for eliminating infected or malignant cells. Moreover, TNFRSF4 signaling is involved in the regulation of T cell-dependent B cell responses, including the production of antibodies .
Recombinant mouse TNFRSF4 is a laboratory-produced version of the protein that is used in various research applications. It is typically produced using recombinant DNA technology, where the gene encoding TNFRSF4 is inserted into an expression vector and introduced into a host cell system, such as bacteria or mammalian cells. The host cells then produce the TNFRSF4 protein, which can be purified and used for experimental purposes .
Recombinant mouse TNFRSF4 is used in studies to investigate the molecular mechanisms of TNFRSF4 signaling, its role in immune responses, and its potential therapeutic applications. For example, researchers use recombinant TNFRSF4 to study its effects on T cell activation, proliferation, and survival. Additionally, recombinant TNFRSF4 is used in the development of therapeutic strategies targeting TNFRSF4 signaling pathways for the treatment of autoimmune diseases, cancer, and other immune-related disorders .
The TNFRSF4-OX40L signaling axis has significant clinical implications. Modulating this pathway has the potential to enhance immune responses against tumors and infections, as well as to regulate autoimmune and inflammatory diseases. Therapeutic agents targeting TNFRSF4 or OX40L are being developed and tested in clinical trials for their efficacy in treating various conditions, including cancer and autoimmune diseases .
