TNFRSF12A Human, sf9
Sf9, Baculovirus cells.
Tumor necrosis factor receptor superfamily member 12A, FN14, CD266 antigen, TweakR, tweak-receptor, Fibroblast growth factor-inducible immediate-early response protein 14, FGF-inducible 14, type I transmembrane protein Fn14.
Greater than 90% as determined by SDS-PAGE.
Description
TNFRSF12A Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 292 amino acids (28-80a.a.) and having a molecular mass of 32.6kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). TNFRSF12A is expressed with a 239 amino acids hIgG- His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
The gene TNFRSF12A, initially identified as Fn14, is an immediate early response gene induced by fibroblast growth factor in mouse NIH 3T3 fibroblasts. The human TNFRSF12A cDNA encodes a type I transmembrane protein of 129 amino acids, featuring a 27-amino acid signal peptide, a 53-amino acid extracellular domain, a 21-amino acid transmembrane domain, and a 28-amino acid cytoplasmic domain. Human and mouse TNFRSF12A share 82% amino acid sequence identity. As the smallest member of the TNF receptor superfamily, TNFRSF12A possesses a single cysteine-rich region within its extracellular domain. The cytoplasmic domain of TNFRSF12A contains a TRAF binding motif capable of binding TRAFs 1, 2, and 3. TNFRSF12A exhibits high-affinity binding to its ligand, TWEAK/TNFSF12A, initiating a signal transduction cascade that elicits various cellular responses depending on the cell type, including cell death, proliferation, and angiogenesis.
Recombinant human TNFRSF12A, produced in Sf9 insect cells using a baculovirus expression system, is a single, glycosylated polypeptide chain. This protein comprises 292 amino acids (residues 28-80a.a.) and has a molecular mass of 32.6 kDa. Notably, on SDS-PAGE, it appears at a size of approximately 28-40 kDa. This discrepancy arises from glycosylation and the presence of a 239-amino acid hIgG-His tag at the C-terminus. The protein undergoes purification using proprietary chromatographic techniques.
The provided TNFRSF12A protein solution has a concentration of 1 mg/ml and is formulated in a buffer consisting of Phosphate Buffered Saline (pH 7.4), 1mM Dithiothreitol (DTT), and 20% glycerol.
For short-term storage (up to 2-4 weeks), the solution should be kept at 4°C. For extended storage, freezing at -20°C is recommended. To ensure optimal protein stability during long-term storage, the addition of a carrier protein like Albumin (HSA or BSA) at a concentration of 0.1% is advisable. It's important to minimize freeze-thaw cycles to prevent protein degradation.
The purity of this protein is determined to be greater than 90% based on SDS-PAGE analysis.
Tumor necrosis factor receptor superfamily member 12A, FN14, CD266 antigen, TweakR, tweak-receptor, Fibroblast growth factor-inducible immediate-early response protein 14, FGF-inducible 14, type I transmembrane protein Fn14.
Sf9, Baculovirus cells.
EQAPGTAPCS RGSSWSADLD KCMDCASCRA RPHSDFCLGC AAAPPAPFRL LWPLEPKSCD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSRDELTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGKHHHH HH.
Product Science Overview
TNFRSF12A is the smallest member of the TNF receptor superfamily . It is a type I transmembrane protein that consists of an extracellular domain, a transmembrane domain, and a cytoplasmic domain . The human recombinant version of this protein, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain containing 292 amino acids .
The gene encoding TNFRSF12A is located on chromosome 16 in humans . The protein is expressed in various tissues, including the heart, placenta, lung, skeletal muscle, kidney, and pancreas . Its expression is highly regulated both in vivo and in vitro .
TNFRSF12A functions as a receptor for the TNF-like weak inducer of apoptosis (TWEAK), which is its ligand . This receptor-ligand interaction is involved in several biological processes:
- Apoptosis: TNFRSF12A can induce apoptosis in certain cell types, although it is considered a weak inducer .
- Angiogenesis: It promotes the formation of new blood vessels, which is essential for wound healing and tissue regeneration .
- Cell Proliferation: TNFRSF12A stimulates the proliferation of endothelial cells, contributing to tissue repair and regeneration .
- Inflammation: It plays a role in inflammatory responses, which are critical for the body’s defense against infections and injuries .
Alterations in the expression or function of TNFRSF12A have been associated with various diseases, including thyroid cancer and ovarian cancer . The protein’s involvement in apoptosis and inflammation makes it a potential target for therapeutic interventions in cancer and other inflammatory diseases .
