TNFRSF12A Human
TNF Ligand Receptor Superfamily Member 12A Human Recombinant
Cat. No.
BT27590
Source
Escherichia Coli.
Synonyms
Tumor necrosis factor receptor superfamily member 12A, FN14, CD266 antigen, TweakR, tweak-receptor, Fibroblast growth factor-inducible immediate-early response protein 14, FGF-inducible 14, type I transmembrane protein Fn14.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
TNFRSF12A Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 5.6 KDa.
The TNFRSF12A is purified by proprietary chromatographic techniques.
The TNFRSF12A is purified by proprietary chromatographic techniques.
Product Specs
Introduction
The gene for TNFRSF12A, initially identified as Fn14, is a fibroblast growth factor-inducible immediate early response gene found in mouse NIH 3T3 fibroblasts. The human TNFRSF12A cDNA encodes a type I transmembrane protein comprising 129 amino acids. This protein consists of a 27-amino acid signal peptide, a 53-amino acid extracellular domain, a 21-amino acid transmembrane domain, and a 28-amino acid cytoplasmic domain. Human and mouse TNFRSF12A share 82% amino acid sequence identity. TNFRSF12 is the smallest member of the TNF receptor superfamily, possessing only a single cysteine-rich region within its extracellular domain. The cytoplasmic domain of TNFRSF12A contains a TRAF binding motif that interacts with TRAF proteins 1, 2, and 3. TNFRSF12A exhibits high affinity for its ligand, TWEAK/TNFSF12A, initiating a signal transduction cascade. The cellular response to this cascade varies depending on the cell type and includes outcomes such as cell death, proliferation, and angiogenesis.
Description
Recombinant human TNFRSF12A, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 53 amino acids. It has a molecular weight of 5.6 kDa. The purification of TNFRSF12A is achieved using proprietary chromatographic methods.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2µm filtered concentrated solution in phosphate-buffered saline (PBS) at pH 7.4.
Solubility
To reconstitute lyophilized TNFRSF12A, it is recommended to dissolve it in sterile 18 MΩ·cm water at a concentration of at least 100 µg/ml. This solution can then be diluted further into other aqueous solutions.
Stability
Lyophilized TNFRSF12A, though stable at room temperature for 3 weeks, should be stored desiccated at a temperature below -18°C. After reconstitution, TNFRSF12A should be stored at 4°C for 2-7 days. For long-term storage, it is recommended to store it at -18°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 97.0% by the following methods:
(a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis.
(b) Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
(a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis.
(b) Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
Biological Activity
The biological activity of TNFRSF12A is evaluated based on its ability to inhibit TWEAK-induced cell death in HT29 cells. The expected 50% effective concentration (ED50) for this effect is in the range of 1.0-5.0 µg/ml when tested in the presence of 1 µg/ml of recombinant human TWEAK.
Synonyms
Tumor necrosis factor receptor superfamily member 12A, FN14, CD266 antigen, TweakR, tweak-receptor, Fibroblast growth factor-inducible immediate-early response protein 14, FGF-inducible 14, type I transmembrane protein Fn14.
Source
Escherichia Coli.
Amino Acid Sequence
EQAPGTAPCS RGSSWSADLD KCMDCASCRA RPHSDFCLGC AAAPPAPFRL LWP.
Product Science Overview
Structure and Expression
TNFRSF12A is a single, non-glycosylated polypeptide chain containing 53 amino acids and has a molecular mass of approximately 5.6 kDa . The gene encoding this protein is located on chromosome 16 in humans . The expression of TNFRSF12A is highly regulated and can be found in various human tissues, including the heart, placenta, lung, skeletal muscle, kidney, and pancreas .
Function
TNFRSF12A serves as a receptor for the cytokine TNFSF12/TWEAK . It plays a crucial role in several biological processes, including:
- Apoptosis: TNFRSF12A is a weak inducer of apoptosis in some cell types .
- Angiogenesis: It promotes the formation of new blood vessels and the proliferation of endothelial cells .
- Cellular Adhesion: TNFRSF12A may modulate cellular adhesion to matrix proteins .
- Inflammatory Reactions: It is involved in inflammatory responses .
- Wound Healing: TNFRSF12A plays a role in the regulation of wound healing .
Recombinant Production
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