TNFA Bovine
Escherichia Coli.
TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.
Sterile filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
TNFA Bovine produced in E.Coli is a single, non-glycosylated polypeptide chain containing 158 amino acids (78-234 a.a.) and having a molecular mass of 17.5kDa.
TNFA is purified by proprietary chromatographic techniques.
Product Specs
TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.
Escherichia Coli.
MLRSSSQASS NKPVAHVVAD INSPGQLRWW DSYANALMAN GVKLEDNQLV VPADGLYLIY SQVLFRGQGC PSTPLFLTHT ISRIAVSYQT KVNILSAIKS PCHRETPEWA EAKPWYEPIY QGGVFQLEKG DRLSAEINLP DYLDYAESGQ VYFGIIAL.
Product Science Overview
Tumor Necrosis Factor-alpha (TNF-α) is a crucial cytokine involved in systemic inflammation and is part of the body’s immune response. It is a member of the TNF superfamily, which consists of various cytokines that can cause cell death (apoptosis). TNF-α is known for its role in inflammation, immune system development, apoptosis, and lipid metabolism .
TNF-α is a pleiotropic molecule, meaning it has multiple effects on different types of cells. It is produced primarily by macrophages, a type of white blood cell that engulfs and digests cellular debris and pathogens. TNF-α can bind to two receptors: TNFR1 and TNFR2. The binding of TNF-α to these receptors can trigger various cellular responses, including cell death, survival, proliferation, and differentiation .
Recombinant bovine TNF-α is a form of TNF-α that has been genetically engineered for use in research and therapeutic applications. This recombinant protein is produced by inserting the gene that encodes bovine TNF-α into a suitable expression system, such as bacteria or yeast, which then produces the protein in large quantities .
Recombinant bovine TNF-α is used extensively in research to study its effects on various cell types and its role in different diseases. For instance, it has been shown to play a significant role in inflammatory diseases in cattle, such as mastitis, which is an inflammation of the mammary gland . By understanding how TNF-α functions, researchers can develop new therapeutic strategies to treat these diseases.
In addition, recombinant bovine TNF-α has been used to study the effects of TNF-α on cell death and inflammatory cytokine kinetics. For example, studies have shown that TNF-α can induce cell death in certain cell types, and this effect can be modulated by using decoy receptors that bind to TNF-α and prevent it from interacting with its natural receptors .
The therapeutic potential of recombinant bovine TNF-α lies in its ability to modulate the immune response. By blocking the activity of TNF-α, it is possible to reduce inflammation and improve outcomes in diseases characterized by excessive inflammation. For example, decoy receptors for TNF-α have been developed and shown to be effective in reducing inflammation in vitro .
