TLR3 Human
Sf9, Baculovirus cells.
Toll Like Receptor 3, Toll-Like Receptor 3, CD283 Antigen, CD283, IIAE2, Toll-like receptor 3.
Greater than 90.0% as determined by SDS-PAGE.
Description
TLR3 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 690 amino acids (23-704 a.a.) and having a molecular mass of 78.5kDa. (Molecular size on SDS-PAGE will appear at approximately 70-100kDa).
TLR3 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Toll-like receptor 3 (TLR3) is a member of the toll-like receptor family and plays a crucial role in recognizing pathogens and activating the innate immune response. TLR3 functions as an innate immune receptor, detecting both microbial molecules and damage-associated molecular patterns released from damaged host cells. It specifically recognizes double-stranded RNA (dsRNA), and its activation triggers the NF-kappaB pathway, leading to the production of type I interferons. TLR3 signaling activation has been linked to protection against brain ischemia induced by ischemic preconditioning and a reduction in reactive astrogliosis. TLR3 is highly expressed in the placenta and pancreas, with limited expression in dendritic cells within the leukocyte population.
TLR3, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain consisting of 690 amino acids (23-704 a.a.). It has a molecular mass of 78.5 kDa. On SDS-PAGE, the molecular size will appear between 70-100 kDa.
This TLR3 protein is expressed with an 8 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
The TLR3 protein solution is provided at a concentration of 0.5 mg/ml. It is formulated in a buffer containing 20% glycerol, 20mM Tris-HCl (pH 8.0), 0.1M NaCl, and 1mM DTT.
To ensure stability during long-term storage, it is advisable to add a carrier protein (0.1% HSA or BSA).
Repeated freezing and thawing of the protein should be avoided.
The purity of TLR3 is determined to be greater than 90.0% using SDS-PAGE analysis.
Toll Like Receptor 3, Toll-Like Receptor 3, CD283 Antigen, CD283, IIAE2, Toll-like receptor 3.
Sf9, Baculovirus cells.
SSTTKCTVSH EVADCSHLKL TQVPDDLPTN ITVLNLTHNQ LRRLPAANFT RYSQLTSLDV GFNTISKLEP ELCQKLPMLK VLNLQHNELS QLSDKTFAFC TNLTELHLMS NSIQKIKNNP FVKQKNLITL DLSHNGLSST KLGTQVQLEN LQELLLSNNK IQALKSEELD IFANSSLKKL ELSSNQIKEF SPGCFHAIGR LFGLFLNNVQ LGPSLTEKLC LELANTSIRN LSLSNSQLST TSNTTFLGLK WTNLTMLDLS YNNLNVVGND SFAWLPQLEY FFLEYNNIQH LFSHSLHGLF NVRYLNLKRS FTKQSISLAS LPKIDDFSFQ WLKCLEHLNM EDNDIPGIKS NMFTGLINLK YLSLSNSFTS LRTLTNETFV SLAHSPLHIL NLTKNKISKI ESDAFSWLGH LEVLDLGLNE IGQELTGQEW RGLENIFEIY LSYNKYLQLT RNSFALVPSL QRLMLRRVAL KNVDSSPSPF QPLRNLTILD LSNNNIANIN DDMLEGLEKL EILDLQHNNL ARLWKHANPG GPIYFLKGLS HLHILNLESN GFDEIPVEVF KDLFELKIID LGLNNLNTLP ASVFNNQVSL KSLNLQKNLI TSVEKKVFGP AFRNLTELDM RFNPFDCTCE SIAWFVNWIN ETHTNIPELS SHYLCNTPPH YHGFPVRLFD TSSCKDSAPF ELLEHHHHHH.
Product Science Overview
Toll-like receptor 3 is a nucleotide-sensing receptor that is primarily activated by double-stranded RNA, a molecular pattern associated with viral infections . Upon recognition of double-stranded RNA, Toll-like receptor 3 induces the activation of transcription factors such as interferon regulatory factor 3 and nuclear factor kappa-light-chain-enhancer of activated B cells. This activation leads to the production of type I interferons and other pro-inflammatory cytokines, which are essential for antiviral defense .
Toll-like receptor 3 is expressed in various tissues, with the highest levels found in the placenta and pancreas. It is also present in a specific subset of leukocytes known as dendritic cells . The receptor is localized to endosomes within these cells, where it encounters and recognizes double-stranded RNA from internalized viral particles .
The primary function of Toll-like receptor 3 is to detect viral infections and initiate an immune response. By recognizing double-stranded RNA, it triggers signaling pathways that lead to the production of type I interferons and other cytokines. These molecules play a critical role in controlling viral replication and spreading by enhancing the antiviral state of neighboring cells .
Toll-like receptor 3 has been implicated in various diseases and conditions. For example, its activation has been shown to have protective effects in mouse models of atherosclerosis and ischemic brain injury . Additionally, Toll-like receptor 3 activation promotes hair follicle regeneration in skin wound healing . However, overactivation of Toll-like receptor 3 can lead to excessive inflammation and has been associated with autoimmune disorders .
