TIMP4 Human

Tissue Inhibitor of Metalloprotease 4 Human Recombinant
Cat. No.
BT10843
Source
Sf9, Baculovirus cells.
Synonyms
TIMP Metallopeptidase Inhibitor 4, Tissue Inhibitor Of Metalloproteinases 4, Tissue Inhibitor Of Metalloproteinase 4, TIMP-4, Metalloproteinase Inhibitor 4.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TIMP4 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 204 amino acids (30-224 a.a.) and having a molecular mass of 23.5kDa (Molecular size on SDS-PAGE will appear at approximately 18-28kDa). 
TIMP4 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Metalloproteinase inhibitor 4 (TIMP4) is a member of a family of secreted proteins that regulate the activation and proteolytic activity of zinc enzymes known as matrix metalloproteinases. Four members of this family have been identified: TIMP1, TIMP2, TIMP3, and TIMP4. TIMP4 is produced by a variety of tissues, including the brain, heart, ovary, and skeletal muscle. Studies suggest that TIMP4 may act as a tumor suppressor against Wilm's tumor, correlates negatively with glioma malignancy, and is present in breast carcinoma cells.
Description
TIMP4, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain containing 204 amino acids (30-224 a.a.) with a molecular mass of 23.5 kDa. The molecular size on SDS-PAGE will appear at approximately 18-28 kDa. TIMP4 is expressed with a 6-amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Sterile filtered, colorless solution.
Formulation
The TIMP4 protein solution (0.25 mg/mL) is supplied in Phosphate Buffered Saline (pH 7.4) with 10% glycerol.
Stability
For short-term storage (2-4 weeks), store at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Greater than 90.0% purity as determined by SDS-PAGE.
Synonyms
TIMP Metallopeptidase Inhibitor 4, Tissue Inhibitor Of Metalloproteinases 4, Tissue Inhibitor Of Metalloproteinase 4, TIMP-4, Metalloproteinase Inhibitor 4.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
ADPCSCAPAH PQQHICHSAL VIRAKISSEK VVPASADPAD TEKMLRYEIK QIKMFKGFEK VKDVQYIYTP FDSSLCGVKL EANSQKQYLL TGQVLSDGKV FIHLCNYIEP WEDLSLVQRE SLNHHYHLNC GCQITTCYTV PCTISAPNEC LWTDWLLERK LYGYQAQHYV CMKHVDGTCS WYRGHLPLRK EFVDIVQPHH HHHH.

Product Science Overview

Introduction

Tissue Inhibitor of Metalloprotease 4 (TIMP-4) is a member of the Tissue Inhibitors of Metalloproteinases (TIMPs) family, which are natural inhibitors of matrix metalloproteinases (MMPs). MMPs are enzymes responsible for the degradation of the extracellular matrix (ECM), playing a crucial role in tissue remodeling and various physiological processes. TIMP-4, like other TIMPs, regulates the activity of MMPs, thereby influencing ECM composition and integrity .

Structure and Expression

Human TIMP-4 is a non-glycosylated polypeptide consisting of 195 amino acids. It shares a high degree of sequence homology with other TIMPs, particularly TIMP-2, with which it is 51% identical at the amino acid level . TIMP-4 is expressed in a variety of tissues, including the heart, kidney, pancreas, colon, testes, brain, and adipose tissue . This restricted expression pattern suggests that TIMP-4 has specific physiological roles in these tissues.

Functions

TIMP-4 is primarily known for its ability to inhibit MMPs, thereby regulating ECM remodeling. However, recent studies have shown that TIMP-4 also has MMP-independent functions. It can influence cell proliferation, apoptosis, and other cellular processes . For instance, TIMP-4 has been implicated in the regulation of smooth muscle cell (SMC) proliferation and lipid metabolism, which are critical factors in the development of atherosclerosis .

Role in Disease

The dysregulation of TIMP-4 has been associated with various diseases, including cancer and cardiovascular diseases. Overproduction of MMPs, which TIMP-4 inhibits, is often linked to cancer progression and metastasis . In cardiovascular diseases, TIMP-4 plays a protective role by preventing the degradation of ECM components and maintaining vascular integrity . For example, the loss of TIMP-4 has been shown to promote atherosclerotic plaque deposition in the abdominal aorta, independent of plasma cholesterol levels .

Therapeutic Potential

Given its role in regulating MMP activity and its involvement in various diseases, TIMP-4 has potential therapeutic applications. By modulating TIMP-4 levels, it may be possible to develop treatments for conditions characterized by excessive ECM degradation, such as cancer and atherosclerosis . Further research is needed to fully understand the mechanisms by which TIMP-4 exerts its effects and to develop targeted therapies.

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