TIMP2 Human, Sf9

Tissue Inhibitor of Metalloprotease 2 Human Recombinant, Sf9
Cat. No.
BT10723
Source
Sf9, Baculovirus cells.
Synonyms
TIMP Metallopeptidase Inhibitor 2, Tissue Inhibitor Of Metalloproteinases 2, CSC-21K, Tissue Inhibitor Of Metalloproteinase 2, TIMP-2, DDC8, Metalloproteinase inhibitor 2.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TIMP2 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 200 amino acids (27-220a.a.) and having a molecular mass of 22.5kDa. 
(Molecular size on SDS-PAGE will appear at approximately 18-28kDa).
TIMP2 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
TIMP2, a member of the TIMP family, is a natural inhibitor of matrix metalloproteinases (MMPs), enzymes involved in extracellular matrix degradation. This protein plays a critical role in tissue homeostasis by suppressing the proliferation of endothelial cells, thereby regulating tissue growth in response to angiogenic factors and controlling protease activity during tissue remodeling.
Description
Recombinant TIMP2, expressed in Sf9 insect cells using baculovirus, is a single, glycosylated polypeptide chain. It encompasses amino acids 27 to 220, resulting in a molecular weight of 22.5 kDa. Note: The apparent molecular size on SDS-PAGE may vary between 18-28 kDa. This TIMP2 variant is engineered with a 6-amino acid His tag at the C-terminus to facilitate purification via proprietary chromatographic methods.
Physical Appearance
The product is a clear, sterile-filtered solution.
Formulation
TIMP2 is supplied as a 0.25 mg/ml solution in Phosphate Buffered Saline (pH 7.4) with 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep the product at 4°C. For extended storage, freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of TIMP2 is greater than 95.0% as determined by SDS-PAGE analysis.
Synonyms
TIMP Metallopeptidase Inhibitor 2, Tissue Inhibitor Of Metalloproteinases 2, CSC-21K, Tissue Inhibitor Of Metalloproteinase 2, TIMP-2, DDC8, Metalloproteinase inhibitor 2.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
CSCSPVHPQQ AFCNADVVIR AKAVSEKEVD SGNDIYGNPI KRIQYEIKQI KMFKGPEKDI EFIYTAPSSA VCGVSLDVGG KKEYLIAGKA EGDGKMHITL CDFIVPWDTL STTQKKSLNH RYQMGCECKI TRCPMIPCYI SSPDECLWMD WVTEKNINGH QAKFFACIKR SDGSCAWYRG AAPPKQEFLD IEDPHHHHHH.

Product Science Overview

Introduction

Tissue Inhibitor of Metalloprotease 2 (TIMP-2) is a member of the TIMP family, which consists of four protease inhibitors: TIMP-1, TIMP-2, TIMP-3, and TIMP-4. These inhibitors play a crucial role in regulating the activity of matrix metalloproteinases (MMPs), which are zinc-dependent endopeptidases involved in the degradation of the extracellular matrix (ECM) and various cell surface proteins .

Structure and Function

TIMP-2 is a secreted protein that binds to MMPs, inhibiting their proteolytic activity. This binding prevents MMPs from accessing their catalytic domain, thereby regulating ECM turnover and maintaining tissue homeostasis . TIMP-2 is unique among the TIMP family members due to its ability to form a complex with MMP-14, which in turn activates pro-MMP-2, highlighting its dual role in both inhibition and activation of MMPs .

Biological Significance

The regulation of MMP activity by TIMP-2 is essential for various physiological processes, including tissue repair, wound healing, angiogenesis, and morphogenesis . An imbalance between TIMPs and MMPs has been linked to several pathological conditions, such as rheumatoid arthritis, cardiovascular diseases, cancer metastasis, and endometriosis .

Recombinant TIMP-2 (Human, Sf9)

Recombinant TIMP-2 (Human, Sf9) is produced using the Sf9 insect cell expression system. This system is widely used for the production of recombinant proteins due to its ability to perform post-translational modifications similar to those in mammalian cells . The recombinant TIMP-2 produced in Sf9 cells retains its biological activity and is used in various research applications, including enzymatic assays and studies on ECM regulation .

Applications in Research

Recombinant TIMP-2 is utilized as a positive control in enzymatic assays to study MMP activity and inhibition . It is also employed in research focused on understanding the mechanisms of ECM turnover and the role of TIMPs in various diseases . Additionally, recombinant TIMP-2 is used in studies investigating the therapeutic potential of TIMPs in treating conditions associated with excessive MMP activity .

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