TIE1 Fc Mouse

The TIE1 Fc Chimera (Mouse Recombinant) is a fusion protein that combines the extracellular domain of the mouse TIE1 receptor with the Fc region of an immunoglobulin. This recombinant protein is used in various research applications, particularly in the study of angiogenesis and vascular biology.

The TIE1 receptor, also known as tyrosine kinase with immunoglobulin and EGF homology domains 1, is a receptor tyrosine kinase that plays a crucial role in vascular development and integrity. The extracellular domain of TIE1 contains:

• Two immunoglobulin-like domains
• Three epidermal growth factor (EGF)-like domains
• Three fibronectin type III-like repeats

These domains are followed by a split tyrosine kinase domain in the cytoplasmic region .

The Fc region of the chimera is derived from an immunoglobulin, typically IgG. This region is responsible for the dimerization of the protein and enhances its stability and half-life in vivo.

The TIE1 Fc Chimera (Mouse Recombinant) is produced using recombinant DNA technology. The gene encoding the extracellular domain of mouse TIE1 is fused with the gene encoding the Fc region of an immunoglobulin. This construct is then expressed in a suitable host cell line, such as a mouse myeloma cell line (NS0) .

The recombinant protein is purified using affinity chromatography techniques to ensure high purity and low endotoxin levels. The final product is typically lyophilized and can be reconstituted in a suitable buffer for experimental use.

The TIE1 receptor is primarily expressed in endothelial cells and some hematopoietic progenitor cells. It plays a significant role in modulating angiopoietin signaling, which is crucial for blood vessel formation and maintenance . Although TIE1 does not directly bind to angiopoietins, it can form heterodimers with TIE2, another receptor tyrosine kinase, to modulate angiopoietin signaling pathways .

The TIE1 Fc Chimera can be used in various in vitro and in vivo assays to study its interactions with other proteins and its role in angiogenesis. It is also used in binding studies, where its ability to bind to integrins and other cell surface receptors is evaluated .

The TIE1 Fc Chimera (Mouse Recombinant) is widely used in research focused on:

• Angiogenesis and vascular biology
• Cancer research, particularly in studying tumor angiogenesis
• Inflammatory diseases, where vascular integrity is compromised
• Developmental biology, to understand the role of TIE1 in vascular development

By using this recombinant protein, researchers can gain insights into the molecular mechanisms underlying these processes and develop potential therapeutic strategies targeting the TIE1 signaling pathway.