Thyroglobulin Human, Biotin

Thyroglobulin Human, Biotinylated
Cat. No.
BT3018
Source
Native, Isolated from human thyroid glands.
Synonyms
Thyroglobulin, TGN, AITD3, TG.
Appearance
Sterile Filtered solution.
Purity

Greater than 80% as determined by SDS-PAGE.

Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Human Thyroglobulin is a biotinylated, glycosylated, polypeptide chain having a total molecular mass of 662 kDa (331 kDa per subunit).

Product Specs

Introduction
Thyroglobulin (TG) is a major autoantigen in human autoimmune thyroid disease. This large, globular, dimeric glycoprotein (660 kDa) is crucial for thyroid hormone biosynthesis. It constitutes about 75% of the thyroid follicle's protein content. Thyroid epithelial cells (thyrocytes) within these follicles produce TG, which is then secreted and stored in the follicular lumen. TG acts as a precursor for the production of the thyroid hormones thyroxine (T4) and triiodothyronine (T3). Individuals with Hashimoto's thyroiditis or Graves' disease often develop antibodies against TG. While these Tg-specific antibodies aid in diagnosing these diseases, they can also be present in seemingly healthy euthyroid individuals. Blood TG levels can serve as a tumor marker for certain thyroid cancers and may be elevated in Graves' disease.
Description
Human Thyroglobulin is a biotinylated and glycosylated polypeptide chain with a total molecular weight of 662 kDa (each subunit weighs 331 kDa).
Physical Appearance
Sterile Filtered solution.
Formulation
Human Thyroglobulin biotinylated is supplied in a solution containing 20mM HEPES buffer (pH 7.6), 150mM NaCl, and 40% Sucrose (w/v).
Stability
For short-term storage (2-4 weeks), keep at 4°C. For longer periods, store frozen at -20°C. Avoid repeated freezing and thawing.
Purity
Purity is greater than 80% as determined by SDS-PAGE.
Immunological Functions
This product has the following immunological functions: 1. Binds to human autoantibodies of the IgG type. 2. Autoantibodies targeting thyroglobulin recognize conformation-dependent epitopes. 3. Can be used in functional Streptavidin-based ELISA tests to analyze positive and negative samples.
Synonyms
Thyroglobulin, TGN, AITD3, TG.
Source
Native, Isolated from human thyroid glands.

Product Science Overview

Structure and Function

Thyroglobulin is synthesized in the thyroid gland and serves as a precursor for the production of T4 and T3. It contains numerous tyrosine residues, which are iodinated to form monoiodotyrosine (MIT) and diiodotyrosine (DIT). These iodinated tyrosines are then coupled to form T4 and T3, which are critical for regulating metabolism, growth, and development .

Biotinylation

Biotinylation refers to the process of attaching biotin (a vitamin) to proteins and other macromolecules. Biotinylated thyroglobulin is used in various biochemical assays, including enzyme-linked immunosorbent assays (ELISA), to detect and quantify the presence of thyroglobulin in biological samples . The biotinylation of thyroglobulin enhances its detection sensitivity and allows for more accurate measurements in research and diagnostic applications .

Applications

Biotinylated thyroglobulin is widely used in research to study thyroid function and disorders. It is also employed in clinical diagnostics to monitor thyroid cancer patients, as elevated levels of thyroglobulin can indicate the presence of residual or recurrent disease . Additionally, it is used in the development of immunoassays and other diagnostic tools to measure thyroid hormone levels and assess thyroid health .

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