Thyroglobulin Human
Mammalian cell line.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 95.0% as determined by SDS-PAGE.
Description
Thyroglobulin Human produced in a mammalian cell line is a single, non-glycosylated polypeptide chain (1-2768 a.a.) and having a molecular mass of 304640 Dalton.
Thyroglobulin Human is fused with GlyAlaProGly4SerHis10-tag at C-terminal and purified by proprietary chromatographic techniques.
Product Specs
Mammalian cell line.
Product Science Overview
Thyroglobulin is a large glycoprotein produced exclusively by the thyroid gland. It plays a crucial role in the synthesis and storage of thyroid hormones. The recombinant form of thyroglobulin, known as human recombinant thyroglobulin, is produced using advanced biotechnological methods to replicate the natural protein’s structure and function.
Thyroglobulin is a dimeric glycoprotein with a molecular weight of approximately 660 kDa. It is composed of a series of tyrosine residues, which serve as the scaffold for the synthesis of thyroid hormones. The primary function of thyroglobulin is to facilitate the organification of iodine and the production of thyroid hormones, including triiodothyronine (T3) and thyroxine (T4) .
Thyroglobulin is synthesized by thyroid follicular cells and stored in the follicular lumen of the thyroid gland. The process begins with the uptake of iodide from the bloodstream, which is then transported to the colloid by the sodium/iodide symporter (NIS) and pendrin . Thyroid peroxidase (TPO) catalyzes the oxidation of iodide, which is then incorporated into thyroglobulin to form monoiodotyrosine (MIT) and diiodotyrosine (DIT). These iodinated tyrosines couple to produce T3 and T4 .
Thyroglobulin measurement is a cornerstone in the management of differentiated thyroid cancer (DTC). It serves as a tumor marker to monitor the presence of residual or recurrent disease after thyroidectomy and radioactive iodine ablation . Elevated levels of thyroglobulin in the bloodstream can indicate the presence of thyroid tissue or malignancy .
Human recombinant thyroglobulin is produced using recombinant DNA technology, which involves inserting the gene encoding thyroglobulin into a suitable expression system, such as bacteria or yeast. This method allows for the large-scale production of thyroglobulin with high purity and consistency . Recombinant thyroglobulin is used in various clinical and research applications, including the development of diagnostic assays and therapeutic interventions .
- Diagnostic Assays: Recombinant thyroglobulin is used in the development of highly sensitive assays for the detection of thyroglobulin levels in patients with thyroid disorders .
- Therapeutic Interventions: It is also explored as a potential therapeutic agent in the treatment of thyroid-related diseases .
- Research: Recombinant thyroglobulin is valuable in research settings to study the biochemical properties and physiological significance of thyroglobulin .
