TDP1 Human, Sf9
Tyrosyl-DNA phosphodiesterase 1, Tyr-DNA phosphodiesterase 1, TDP1, FLJ11090, MGC104252.
Greater than 90.0% as determined by SDS-PAGE.
Description
TDP1 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 617 amino acids (1-608) and having a molecular mass of 69.5kDa. TDP1 is fused to 6 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
Tyrosyl-DNA phosphodiesterase 1, Tyr-DNA phosphodiesterase 1, TDP1, FLJ11090, MGC104252.
ADPMSQEGDY GRWTISSSDE SEEEKPKPDK PSTSSLLCAR QGAANEPRYT CSEAQKAAHK RKISPVKFSN TDSVLPPKRQ KSGSQEDLGW CLSSSDDELQ PEMPQKQAEK VVIKKEKDIS APNDGTAQRT ENHGAPACHR LKEEEDEYET SGEGQDIWDM LDKGNPFQFY LTRVSGVKPK YNSGALHIKD ILSPLFGTLV SSAQFNYCFD VDWLVKQYPP EFRKKPILLV HGDKREAKAH LHAQAKPYEN ISLCQAKLDI AFGTHHTKMM LLLYEEGLRV VIHTSNLIHA DWHQKTQGIW LSPLYPRIAD GTHKSGESPT HFKADLISYL MAYNAPSLKE WIDVIHKHDL SETNVYLIGS TPGRFQGSQK DNWGHFRLKK LLKDHASSMP NAESWPVVGQ FSSVGSLGAD ESKWLCSEFK ESMLTLGKES KTPGKSSVPL YLIYPSVENV RTSLEGYPAG GSLPYSIQTA EKQNWLHSYF HKWSAETSGR SNAMPHIKTY MRPSPDFSKI AWFLVTSANL SKAAWGALEK NGTQLMIRSY ELGVLFLPSA FGLDSFKVKQ KFFAGSQEPM ATFPVPYDLP PELYGSKDRP WIWNIPYVKA PDTHGNMWVP SHHHHHH.
Product Science Overview
TDP1 belongs to the phospholipase D family and contains two PLD phosphodiesterase domains . The enzyme’s primary function is to repair topoisomerase I-mediated DNA damage by catalyzing the hydrolysis of the phosphodiester bond . This activity is critical for maintaining genomic stability and preventing the accumulation of DNA damage that can lead to various diseases, including cancer .
The recombinant form of TDP1, produced in Sf9 cells, is used for research and therapeutic purposes. Sf9 cells, derived from the fall armyworm (Spodoptera frugiperda), are commonly used in the baculovirus expression system for producing recombinant proteins . This system allows for high-level expression of functional proteins with post-translational modifications similar to those in higher eukaryotes .
Mutations in the TDP1 gene are associated with spinocerebellar ataxia with axonal neuropathy (SCAN1), a rare neurodegenerative disorder . This disease is characterized by progressive ataxia, peripheral neuropathy, and other neurological symptoms . Understanding the function and regulation of TDP1 is crucial for developing potential therapeutic strategies for SCAN1 and other conditions involving DNA damage and repair .
