TDP1 Antibody
Description
Product Specs
Product Science Overview
Tyrosyl-DNA phosphodiesterase 1 (TDP1) is an enzyme encoded by the TDP1 gene in humans. This enzyme plays a crucial role in the repair of DNA damage, specifically by hydrolyzing the phosphodiester bond between the tyrosine residue of Type I topoisomerase and the 3-prime phosphate of DNA . This action is essential for resolving stalled topoisomerase I-DNA complexes, which can otherwise lead to DNA damage and genomic instability .
TDP1 is a member of the phospholipase D family and contains two PLD phosphodiesterase domains . It is involved in the repair of single-strand breaks and double-strand breaks in DNA, making it a key player in maintaining genomic integrity . Mutations in the TDP1 gene are associated with the disease spinocerebellar ataxia with axonal neuropathy (SCAN1), highlighting its importance in neural function and stability .
Mouse anti-human antibodies are secondary antibodies generated by immunizing mice with human immunoglobulins. These antibodies are affinity-purified and have well-characterized specificity for human immunoglobulins . They are commonly used in various detection systems, including enzyme-linked immunosorbent assays (ELISA), Western blotting, immunohistochemistry (IHC), and flow cytometry .
Secondary antibodies, such as mouse anti-human antibodies, offer increased versatility and sensitivity through signal amplification. This is because multiple secondary antibodies can bind to a single primary antibody, enhancing the detection signal . These antibodies are essential tools in research and diagnostic applications, enabling the detection, sorting, and purification of specific target proteins or cells .
The combination of TDP1 and mouse anti-human antibodies can be particularly useful in research focused on DNA repair mechanisms and the study of diseases associated with DNA damage. For instance, researchers can use mouse anti-human antibodies to detect and quantify TDP1 in various samples, facilitating the study of its role in DNA repair and its implications in diseases like SCAN1 .
