TDG Human
Greater than 85% as determined by SDS-PAGE.
Description
TDG is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Thymine-DNA glycosylase (TDG) is a crucial enzyme involved in the base excision repair (BER) pathway, which is responsible for maintaining genomic stability by correcting DNA base mismatches. This enzyme is particularly significant in the context of human health, as it plays a vital role in preventing mutations that could lead to diseases such as cancer.
TDG belongs to the TDG/mug DNA glycosylase family and is characterized by its ability to remove thymine moieties from G/T mismatches. It achieves this by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine . Additionally, TDG can excise uracil and 5-bromouracil from mispairings with guanine, albeit with lower activity .
The enzyme operates through a base-flipping mechanism, where the target base is flipped out of the DNA helix and into the enzyme’s active site. This allows TDG to recognize and bind to the substrate, catalyzing the hydrolysis of the N-glycosidic bond and generating an abasic site (AP-site) in the DNA backbone .
TDG plays a central role in the cellular defense against genetic mutations. It is particularly important in countering the effects of spontaneous deamination of 5-methylcytosine and cytosine, which can lead to G/T and G/U mismatches, respectively . By excising these mismatched bases, TDG helps to maintain the integrity of the genetic code and prevent the accumulation of mutations that could potentially lead to cancer and other genetic disorders.
Recombinant human TDG is produced using recombinant DNA technology, which involves inserting the human TDG gene into a suitable expression system, such as bacteria or yeast. This allows for the large-scale production of the enzyme, which can then be purified and used for various research and therapeutic applications .
Recombinant TDG is particularly valuable in studying the enzyme’s structure and function, as well as its role in DNA repair mechanisms. It also holds potential for therapeutic applications, such as enhancing DNA repair in cancer cells to improve the efficacy of certain treatments.
