TCF4 Human, sf9
Transcription Factor 4 Human Recombinant, sf9
Cat. No.
BT23426
Source
Sf9, Baculovirus cells.
Synonyms
Transcription Factor 4, Class B Basic Helix-Loop-Helix
Protein 19, Immunoglobulin Transcription Factor 2, SL3-3 Enhancer Factor 2,
BHLHb19, ITF-2, SEF-2, TCF-4, ITF2, SEF2, SEF2-1A, SEF2-1B, SEF2-1D, SEF2-1,
FECD3, E2-2, PTHS.
Appearance
Sterile Filtered colorless solution.
Purity
Greater
than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
TCF4 produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 513 amino acids (1-507a.a.) and
having a molecular mass of 54.9kDa (Molecular size on SDS-PAGE will appear at
approximately 35-100kDa). TCF4 is expressed with a 6 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
Transcription Factor 4 (TCF4) is a transcription factor involved in initiating neuronal differentiation. It recognizes and binds to the Ephrussi-box ('E-box') binding site ('CANNTG'), a motif initially identified in immunoglobulin enhancers. TCF4 is primarily expressed in pre-B-cells, although it is also found in other tissues.
Description
Produced in Sf9 Baculovirus cells, TCF4 is a single, glycosylated polypeptide chain consisting of 513 amino acids (1-507a.a.) with a molecular mass of 54.9kDa. Notably, its molecular size on SDS-PAGE appears between 35-100kDa. The protein is expressed with a 6 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The TCF4 protein solution is provided at a concentration of 0.5mg/ml and contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the protein should be stored at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of TCF4 is greater than 85.0%, as determined by SDS-PAGE analysis.
Synonyms
Transcription Factor 4, Class B Basic Helix-Loop-Helix
Protein 19, Immunoglobulin Transcription Factor 2, SL3-3 Enhancer Factor 2,
BHLHb19, ITF-2, SEF-2, TCF-4, ITF2, SEF2, SEF2-1A, SEF2-1B, SEF2-1D, SEF2-1,
FECD3, E2-2, PTHS.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
MKFKQCRCSD TGLCCLDHEG KAEVYAPSAS TADYNRDSPG YPSSKPATST
FPSSFFMQDG HHSSDPWSSS SGMNQPGYAG MLGNSSHIPQ SSSYCSLHPH ERLSYPSHSS ADINSSLPPM
STFHRSGTNH YSTSSCTPPA NGTDSIMANR GSGAAGSSQT GDALGKALAS IYSPDHTNNS FSSNPSTPVG SPPSLSAGTA VWSRNGGQAS SSPNYEGPLH SLQSRIEDRL
ERLDDAIHVL RNHAVGPSTA MPGGHGDMHG IIGPSHNGAM GGLGSGYGTG LLSANRHSLM VGTHREDGVA
LRGSHSLLPN QVPVPQLPVQ SATSPDLNPP QDPYRGMPPG LQGQSVSSGS SEIKSDDEGD ENLQDTKSSE DKKLDDDKKD
IKSITSNNDD EDLTPEQKAE REKERRMANN ARERLRVRDI NEAFKELGRM VQLHLKSDKP QTKLLILHQA VAVILSLEQQ
VRERNLNPKA ACLKRREEEK VSSEPPPLSL AGPHPGMGDA SNHMGQMHHH HHH.
Product Science Overview
Recombinant Production in Sf9 Cells
The recombinant production of TCF4 in Sf9 cells utilizes the baculovirus expression vector system (BEVS). Sf9 cells, derived from the fall armyworm (Spodoptera frugiperda), are commonly used in BEVS due to their ability to grow in suspension cultures and serum-free media, making them suitable for large-scale protein production .
The process involves the following steps:
- Cloning of TCF4 Gene: The TCF4 gene is cloned into a baculovirus transfer vector.
- Transfection: The recombinant baculovirus is used to transfect Sf9 cells.
- Expression: Infected Sf9 cells express the TCF4 protein.
- Purification: The recombinant TCF4 protein is purified from the cell culture supernatant.
Applications and Significance
Recombinant TCF4 produced in Sf9 cells is used in various research applications, including:
- Neuroscience Research: Studying the role of TCF4 in neuronal differentiation and brain development.
- Immunology: Investigating TCF4’s involvement in immune cell function and response.
- Gene Regulation Studies: Understanding how TCF4 regulates target genes through E-box binding.
The availability of human recombinant TCF4 facilitates these studies by providing a consistent and scalable source of the protein .
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