TAGLN3 Human

Transgelin-3 Human Recombinant

Cat. No.

BT16370

Source

E.coli.

Synonyms

Transgelin 3, NP22, NP25, Neuronal protein 22, Neuronal protein NP25.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 90% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

TAGLN3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 219 amino acids (1-199) and having a molecular mass of 24.6 kDa.
The TAGLN3 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction

TAGLN3 is a protein that contains several important domains: a potential Actin-binding domain, two possible phosphorylation sites, two EF-hand motifs, and a calponin-homology (CH) domain. It shares similarities with transgelin and calponin, both proteins known to interact with the cytoskeleton. Belonging to the calponin family, TAGLN3 co-localizes with Actin and tubulin, suggesting its involvement in neuronal plasticity or signal transduction. Its widespread expression pattern points to diverse roles within the developing and mature brain.

Description

This product consists of recombinant human TAGLN3 protein produced in E. coli. It is a single, non-glycosylated polypeptide chain comprising 219 amino acids (residues 1-199) with a molecular weight of 24.6 kDa. For purification, a 20 amino acid His-Tag is fused to the N-terminus, and the protein is purified using proprietary chromatographic methods.

Physical Appearance

Clear, colorless, and sterile-filtered solution.

Formulation

The TAGLN3 solution is provided at a concentration of 0.5mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 1mM DTT, and 20% glycerol.

Stability

For short-term storage (2-4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the protein at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for extended storage durations. Avoid repeated freeze-thaw cycles.

Purity

The purity of the protein is determined to be greater than 90% using SDS-PAGE analysis.

Synonyms

Transgelin 3, NP22, NP25, Neuronal protein 22, Neuronal protein NP25.

Source

E.coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MANRGPSYGL SREVQEKIEQ KYDADLENKL VDWIILQCAE DIEHPPPGRA HFQKWLMDGT VLCKLINSLY PPGQEPIPKI SESKMAFKQM EQISQFLKAA ETYGVRTTDI FQTVDLWEGK DMAAVQRTLM ALGSVAVTKD DGCYRGEPSW FHRKAQQNRR GFSEEQLRQG QNVIGLQMGS NKGASQAGMT GYGMPRQIM

Product Science Overview

Biological Properties

Transgelin-3 shares homology with other cytoskeleton-interacting proteins such as transgelin and calponin. This homology suggests that Transgelin-3 may play a role in cytoskeletal dynamics and cellular motility . The protein is known to co-localize with actin and tubulin, indicating its potential involvement in maintaining the structural integrity of the cytoskeleton.

Expression Patterns and Tissue Distribution

Transgelin-3 exhibits a varied expression pattern, which implies that it may have different roles in the developing and adult brain. Its expression is particularly noted in neuronal tissues, suggesting a specialized function in the nervous system.

Biological Functions

The exact biological functions of Transgelin-3 are still under investigation. However, its interaction with actin and tubulin hints at a role in neuronal plasticity or as a signaling protein. This interaction is crucial for maintaining the dynamic nature of the cytoskeleton, which is essential for various cellular processes, including cell shape, motility, and division.

Modes of Action

Transgelin-3’s actin-binding domain allows it to interact directly with actin filaments, potentially influencing their organization and stability. The EF-hand motifs and phosphorylation sites suggest that its activity could be regulated by calcium ions and phosphorylation events, respectively. These regulatory mechanisms are vital for the protein’s function in response to cellular signals and environmental changes.

Regulatory Mechanisms

The regulation of Transgelin-3 involves multiple layers, including transcriptional control, post-translational modifications, and interactions with other cytoskeletal proteins. The presence of phosphorylation sites indicates that kinases and phosphatases may modulate its activity, thereby influencing its role in the cell.

Recombinant Human Transgelin-3

Recombinant human Transgelin-3 is typically produced in E. coli and purified using conventional chromatography techniques . The recombinant protein often includes an N-terminal His-tag to facilitate purification and detection . It is used in various research applications to study the protein’s function, interactions, and regulatory mechanisms .

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TAGLN3 Human

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