STX6 Human

Syntaxin-6 Human Recombinant
Cat. No.
BT8982
Source
Escherichia Coli.
Synonyms
Syntaxin 6.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

STX6 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 257 amino acids (1-234) and having a molecular mass of 29.2kDa.
STX6 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
STX6, a member of the syntaxin family, possesses a single t-SNARE coiled-coil homology domain and participates in intracellular vesicle trafficking. It interacts with various proteins including EEA1, VPS45A, MARCH2, MARCH3, and GOPC. STX6 is also found within a complex composed of STX6, STX12, VAMP4, and VTI1A.
Description
Recombinant human STX6, expressed in E. coli, is a non-glycosylated polypeptide chain with a molecular weight of 29.2 kDa. The protein consists of 257 amino acids, with residues 1-234 corresponding to the STX6 sequence. A 23 amino acid His-tag is fused to the N-terminus to facilitate purification, which is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear solution that has undergone sterile filtration.
Formulation
The STX6 solution is provided at a concentration of 0.5 mg/ml and is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.2 M NaCl, 2 mM DTT, and 50% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the product in frozen form at -20°C. The addition of a carrier protein, such as 0.1% HSA or BSA, is advised for long-term storage. Repeated freezing and thawing of the product should be avoided.
Purity
SDS-PAGE analysis indicates a purity level exceeding 90.0%.
Synonyms
Syntaxin 6.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSMEDPF FVVKGEVQKA VNTAQGLFQR WTELLQDPST ATREEIDWTT NELRNNLRSI EWDLEDLDET ISIVEANPRK FNLDATELSI RKAFITSTRQ VVRDMKDQMS TSSVQALAER KNRQALLGDS GSQNWSTGTT DKYGRLDREL QRANSHFIEE QQAQQQLIVE QQDEQLELVS GSIGVLKNMS QRIGGELEEQ AVMLEDFSHE LESTQSRLDN VMKKLAKVSH MTSDRRQ

Product Science Overview

Structure and Localization

Syntaxin-6 is characterized by a single-pass type IV membrane protein structure and contains a t-SNARE coiled-coil homology domain . It is predominantly localized in the trans-Golgi network and endosomes, where it plays a pivotal role in the sorting and transport of proteins . The protein is also found in the plasma membrane, contributing to its diverse functional roles within the cell .

Functional Role

The primary function of Syntaxin-6 involves the regulation of intracellular vesicle trafficking. It interacts with other SNARE proteins, such as STX12, VAMP4, and VTI1A, to form a complex that facilitates the fusion of vesicles with target membranes . This interaction is essential for the proper delivery of cargo proteins to their designated locations within the cell.

Clinical Significance

Syntaxin-6 has been implicated in various pathological conditions, particularly in the context of cancer. Overexpression of STX6 has been observed in several types of human malignant tumors, including esophageal, colorectal, and renal cell carcinomas . Recent studies have highlighted its role in hepatocellular carcinoma (HCC), where it promotes tumor progression by enhancing the activation of the JAK-STAT signaling pathway . This makes Syntaxin-6 a potential therapeutic target for cancer treatment.

Recombinant Human Syntaxin-6

Recombinant human Syntaxin-6 is produced using conventional chromatography techniques and is typically expressed in E. coli . The recombinant protein is often tagged with a His-tag at the N-terminus to facilitate purification and detection. It is used extensively in research to study the protein’s function, interactions, and role in disease mechanisms .

Applications in Research

Recombinant human Syntaxin-6 is utilized in various experimental applications, including:

  • Protein-Protein Interaction Studies: To investigate the interactions between Syntaxin-6 and other SNARE proteins or regulatory factors.
  • Functional Assays: To assess the role of Syntaxin-6 in vesicle trafficking and membrane fusion events.
  • Cancer Research: To explore the involvement of Syntaxin-6 in tumorigenesis and its potential as a therapeutic target.

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