STX4 Human

Syntaxin-4 Human Recombinant
Cat. No.
BT8885
Source
E.coli.
Synonyms
Syntaxin 4, syntaxin 4A (placental), STX4A, Renal carcinoma antigen NY-REN-31, p35-2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

STX4 Human Recombinant produced in E. coli is a single polypeptide chain containing 300 amino acids (1-275) and having a molecular mass of 34.7 kDa.
STX4 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Syntaxin 4 (STX4) is a protein belonging to the syntaxin/epimorphin family. Syntaxin proteins act as cellular receptors for transport vesicles, playing a crucial role in exocytosis within neutrophils. STX4 is essential for maintaining normal glucose metabolism regulation in skeletal muscle. Reduced STX4 protein levels can impair hormone-stimulated glucose metabolism throughout the body. This protein is found in neutrophils and HL-60 cells differentiated into neutrophils, with expression levels increasing upon differentiation.
Description
Recombinant human STX4, produced in E. coli, is a single polypeptide chain consisting of 300 amino acids (residues 1-275). It has a molecular weight of 34.7 kDa. The protein includes a 25 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A sterile, colorless solution.
Formulation
The STX4 solution (1mg/ml) is supplied in a buffer containing 20mM Tris-HCl (pH 8.0), 100mM NaCl, 1mM DTT, and 40% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of the protein is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
Syntaxin 4, syntaxin 4A (placental), STX4A, Renal carcinoma antigen NY-REN-31, p35-2.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMMRDRT HELRQGDDSS DEEDKERVAL VVHPGTARLG SPDEEFFHKV RTIRQTIVKL GNKVQELEKQ QVTILATPLP EESMKQELQN LRDEIKQLGR EIRLQLKAIE PQKEEADENY NSVNTRMRKT QHGVLSQQFV ELINKCNSMQ SEYREKNVER IRRQLKITNA GMVSDEELEQ MLDSGQSEVF VSNILKDTQV TRQALNEISA RHSEIQQLER SIRELHDIFT FLATEVEMQG EMINRIEKNI LSSADYVERG QEHVKTALEN QKKARKKKVL

Product Science Overview

Structure and Function

Syntaxin-4 is widely expressed in various tissues and cells. The human Syntaxin-4 protein consists of 297 amino acids and includes several important domains and motifs :

  • Basolateral targeting motif: Located at amino acids 24-29, this motif is crucial for targeting the protein to the basolateral membrane in epithelial cells.
  • Coiled-coil domain (Habc): Spanning amino acids 43-163, this domain is involved in protein-protein interactions.
  • S-nitrosylation site: Found at amino acid 141, this site regulates the fusion activity of Syntaxin-4.
  • t-SNARE domain: Located at amino acids 200-262, this domain is essential for the formation of the SNARE complex.
  • C-terminal transmembrane domain: Spanning amino acids 276-296, this domain anchors the protein to the membrane.
Mechanism of Action

In the process of membrane fusion, Syntaxin-4 interacts with other SNARE proteins, including VAMPs (Vesicle-Associated Membrane Proteins) and SNAPs (Soluble NSF Attachment Proteins) . These interactions facilitate the fusion of vesicles with target membranes, allowing the transfer of cargo between cellular compartments. SM (Sec1/Munc18-like) accessory proteins act as catalysts in this process .

Recombinant Human Syntaxin-4

Recombinant human Syntaxin-4 is produced using E. coli expression systems. The recombinant protein typically includes a C-terminal 6-His tag for purification purposes . It is available in various formulations, including carrier-free versions that do not contain Bovine Serum Albumin (BSA), which can interfere with certain applications .

Applications

Recombinant human Syntaxin-4 is used in various research applications, including:

  • Cell adhesion studies: It has been shown to induce adhesion of ATDC5 mouse chondrogenic cells .
  • Protein-protein interaction studies: The recombinant protein can be used to study interactions with other SNARE proteins and regulatory factors.
  • Membrane fusion assays: Researchers use recombinant Syntaxin-4 to investigate the mechanisms of vesicle fusion and exocytosis.
Storage and Stability

Recombinant human Syntaxin-4 is typically lyophilized from a filtered solution in PBS and TCEP. It should be reconstituted at a concentration of 250 μg/mL in PBS . The protein is stable for up to 12 months when stored at -20 to -70°C, and for shorter periods at 2 to 8°C under sterile conditions after reconstitution .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.