STX2 Human

Syntaxin-2 Human Recombinant
Cat. No.
BT8708
Source
E.coli.
Synonyms
Syntaxin 2, epimorphin, EPM, EPIM, STX2A, STX2B, STX2C.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

STX2 Human Recombinant produced in E. coli is a single polypeptide chain containing 289 amino acids (1-264) and having a molecular mass of 33.6 kDa.
STX2 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Syntaxin-2 (STX2), a member of the syntaxin/epimorphin protein family, serves as a cellular receptor for transport vesicles involved in neutrophil exocytosis. STX2 plays a crucial role in regulating epithelial-mesenchymal interactions, epithelial cell morphogenesis, and activation. This t-SNARE protein is primarily localized to the apical plasma membrane and intracellular vesicular structures. Notably, STX2, in conjunction with SNAP-23, is essential for the regulated secretion of surfactant.
Description
Recombinant human STX2, produced in E. coli, is a single polypeptide chain consisting of 289 amino acids (residues 1-264) with a molecular weight of 33.6 kDa. The protein features a 25 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The STX2 solution is provided at a concentration of 0.25 mg/ml in a buffer composed of 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 1 mM DTT, and 50% glycerol.
Stability
For short-term storage (2-4 weeks), the solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of STX2 is greater than 95% as determined by SDS-PAGE analysis.
Synonyms
Syntaxin 2, epimorphin, EPM, EPIM, STX2A, STX2B, STX2C.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMMRDRL PDLTACRKND DGDTVVVVEK DHFMDDFFHQ VEEIRNSIDK ITQYVEEVKK NHSIILSAPN PEGKIKEELE DLNKEIKKTA NKIRAKLKAI EQSFDQDESG NRTSVDLRIR RTQHSVLSRK FVEAMAEYNE AQTLFRERSK GRIQRQLEIT GRTTTDDELE EMLESGKPSI FTSDIISDSQ ITRQALNEIE SRHKDIMKLE TSIRELHEMF MDMAMFVETQ GEMINNIERN VMNATDYVEH AKEETKKAIK YQSKARRKK

Product Science Overview

Gene and Protein Information

The STX2 gene encodes the Syntaxin-2 protein. This gene is involved in various cellular processes, including epithelial-mesenchymal interactions and epithelial cell morphogenesis and activation . The gene is located on chromosome 12 and has several aliases, including STX2A, STX2B, and STX2C .

Function and Mechanism

Syntaxin-2 is essential for epithelial morphogenesis and may mediate calcium-regulated exocytosis during the acrosomal reaction in sperm . Intracellularly, it functions as a vesicle fusion protein, facilitating the fusion of vesicles with target membranes . Extracellularly, it is active in morphogenesis, influencing the adhesion and survival of epithelial cells .

Recombinant Human Syntaxin-2

Recombinant human Syntaxin-2 is produced using E. coli expression systems. The protein is purified to a high degree, with a purity of over 90% as determined by SDS-PAGE under reducing conditions . It is used in various research applications, including studies on cell adhesion, morphogenesis, and vesicle fusion .

Clinical and Research Applications

Syntaxin-2 is associated with several diseases, including acute diarrhea and kernicterus due to isoimmunization . It is also involved in pathways related to nicotine pharmacodynamics and Sertoli-Sertoli cell junction dynamics . Research on recombinant human Syntaxin-2 helps in understanding these pathways and developing potential therapeutic interventions.

Storage and Stability

Recombinant human Syntaxin-2 is typically lyophilized from a filtered solution and can be reconstituted in PBS. It is stable for up to 12 months when stored at -20 to -70°C and for up to 3 months after reconstitution under sterile conditions .

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