Streptavidin Antibody
Description
Product Specs
Anti Streptavidin mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant Streptavidin amino acids 25-183 purified from E. coli.
Product Science Overview
Streptavidin is a tetrameric protein derived from the bacterium Streptomyces avidinii. It is known for its high affinity binding to biotin (vitamin B7), with a dissociation constant (Kd) of approximately (10^{-14}) mol/L . This strong binding affinity has made streptavidin a crucial component in various biochemical and diagnostic applications.
Streptavidin consists of four identical subunits, each capable of binding one biotin molecule. This tetrameric structure allows for multiple biotin-binding events, making it highly effective in applications requiring signal amplification. The protein’s high stability and resistance to denaturation under extreme pH and temperature conditions further enhance its utility in laboratory settings .
Mouse antibodies against streptavidin are monoclonal antibodies produced by hybridizing mouse myeloma cells with spleen cells from immunized mice. These antibodies are highly specific to streptavidin and do not cross-react with avidin, a similar protein found in egg whites . The monoclonal nature of these antibodies ensures consistent performance in various assays.
- Immunohistochemistry and Immunocytochemistry: Mouse anti-streptavidin antibodies are used to localize streptavidin in tissue sections and cell preparations. They can amplify signals in immunohistochemical staining by binding to streptavidin-biotin complexes .
- ELISA and Western Blotting: These antibodies are employed in enzyme-linked immunosorbent assays (ELISA) and western blotting to detect biotinylated proteins. The high affinity binding of streptavidin to biotin ensures sensitive and specific detection .
- Flow Cytometry: Fluorescently labeled streptavidin is used in flow cytometry to visualize biotinylated primary antibodies. This method is particularly useful for multiplexing panels and detecting low-expressing antigens .
The production of mouse anti-streptavidin antibodies involves several steps:
- Immunization: Mice are immunized with recombinant streptavidin protein to elicit an immune response.
- Hybridoma Formation: Spleen cells from the immunized mice are fused with myeloma cells to create hybridoma cells capable of producing monoclonal antibodies.
- Screening and Selection: Hybridoma cells are screened for the production of antibodies specific to streptavidin. Positive clones are selected and expanded.
- Purification: The monoclonal antibodies are purified using protein A affinity chromatography to ensure high purity and specificity .
