STK16 Antibody
Serine/Threonine Kinase 16, Mouse Anti Human
Cat. No.
BT26209
Source
Synonyms
Serine/threonine-protein kinase 16, Myristoylated and
palmitoylated serine/threonine-protein kinase, MPSK, Protein kinase PKL12,
TGF-beta-stimulated factor 1, TSF-1, Tyrosine-protein kinase STK16, hPSK,
STK16, MPSK1, PKL12, TSF1, KRCT.
Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Product Specs
Introduction
Serine/threonine-protein kinase 16 (STK16) is a membrane-associated protein kinase that phosphorylates serine and threonine residues. It plays a role in secretory vesicle trafficking and intracellular signaling. Additionally, STK16 might regulate stromal-epithelial interactions during mammary gland ductal morphogenesis. While STK16 exhibits autophosphorylation on tyrosine residues, its tyrosine-protein kinase activity towards other proteins remains unclear. It may also be involved in TGF-beta signaling.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
Supplied at 1 mg/ml in PBS, pH 7.4, with 10% glycerol and 0.02% sodium azide.
Storage Procedures
For up to 1 month, store at 4°C. For longer-term storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
Stable for 12 months at -20°C and 1 month at 4°C.
Applications
This antibody has been validated for ELISA and Western blot analysis, ensuring its specificity and reactivity. However, optimal working dilutions should be determined empirically for each application. A starting dilution of 1:1000 is recommended.
Synonyms
Serine/threonine-protein kinase 16, Myristoylated and
palmitoylated serine/threonine-protein kinase, MPSK, Protein kinase PKL12,
TGF-beta-stimulated factor 1, TSF-1, Tyrosine-protein kinase STK16, hPSK,
STK16, MPSK1, PKL12, TSF1, KRCT.
Purification Method
STK16 antibody was purified from mouse ascitic fluids by
protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT4A1AT.
Immunogen
Anti-human STK16 mAb,
clone PAT4A1AT, is derived from hybridization of mouse F0 myeloma cells with
spleen cells from BALB/c mice immunized with a recombinant human STK16 protein 1-305
amino acids purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and k light chain.
Product Science Overview
Serine/Threonine Kinase 16 (STK16)
Serine/Threonine Kinase 16 (STK16), also known as Krct, PKL12, MPSK1, or TSF-1, is a myristoylated and palmitoylated serine/threonine protein kinase. It is ubiquitously expressed and conserved among all eukaryotes . STK16 is a member of the Numb-associated Kinase (NAK) family and has an atypical activation loop architecture .
Molecular Characteristics and Functions
STK16 is primarily localized to the Golgi apparatus and is involved in various cellular processes, including:
- TGF-β signaling pathway: STK16 plays a role in the regulation of this pathway, which is crucial for cell growth, proliferation, differentiation, and apoptosis .
- Protein secretion and sorting: It participates in the trans-Golgi network (TGN) protein secretion and sorting .
- Cell cycle regulation: STK16 is involved in the regulation of the cell cycle and Golgi assembly .
Post-Translational Modifications
STK16 undergoes several post-translational modifications, including:
- Fatty acylation: Myristoylation and palmitoylation are essential for its membrane association and function .
- Phosphorylation: This modification is crucial for its kinase activity and regulation .
Interactors
STK16 interacts with various proteins, including GlcNAcK, DRG1, MAL2, Actin, and WDR1, which are involved in different cellular processes .
Mouse Anti-Human Antibodies
Mouse anti-human antibodies are monoclonal antibodies produced by immunizing mice with human antigens. These antibodies are widely used in research, diagnostics, and therapeutics due to their high specificity and affinity for human proteins .
Production and Humanization
The production of mouse anti-human antibodies involves several steps:
- Immunization: Mice are immunized with the target human antigen to elicit an immune response .
- Hybridoma Technology: B cells from the immunized mice are fused with myeloma cells to create hybridomas that produce monoclonal antibodies .
- Screening and Selection: Hybridomas are screened for the production of antibodies with the desired specificity and affinity .
To reduce the immunogenicity of mouse antibodies in human patients, several humanization techniques are employed:
- Chimeric Antibodies: These antibodies contain mouse variable regions and human constant regions .
- Humanized Antibodies: The complementarity-determining regions (CDRs) from mouse antibodies are grafted onto human antibody frameworks .
- Fully Human Antibodies: These are produced using transgenic mice that express human antibody genes or phage display libraries .
Applications
Mouse anti-human antibodies have numerous applications, including:
- Research: They are used to study protein function, localization, and interactions in various biological systems .
- Diagnostics: These antibodies are employed in diagnostic assays to detect specific human proteins or biomarkers .
- Therapeutics: Humanized and fully human antibodies are used as therapeutic agents to treat various diseases, including cancer, autoimmune disorders, and infectious diseases .
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