STC 1 Human
Stanniocalcin-1 Human Recombinant
Cat. No.
BT20230
Source
293 cell line (Human embryonic kidney).
Synonyms
Stanniocalcin-1, STC, STC-1.
Appearance
White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Stanniocalcin-1 Human Recombinant produced in 293 cell line is a single, glycosylated, polypeptide chain containing 240 amino acids and having a total molecular mass of 25.9 kDa. The Stanniocalcin contains 10 residues form the C-Terminal Flag- tag.
Stanniocalcin is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Stanniocalcin 1 (STC1), initially discovered in fish as a regulator of calcium and phosphate, functions differently in mammals. Instead of acting as a hormone, it operates locally within tissues, exhibiting diverse effects. While found in various tissues, it's usually absent in the bloodstream under normal conditions, likely due to binding with specific proteins. STC1 influences calcium balance, bone and muscle development, and blood vessel formation by interacting with various cell types in mice. Notably, its levels in the heart muscle are altered during heart failure, potentially affecting calcium handling in heart cells. Furthermore, STC1 selectively controls the growth and organization of blood vessels stimulated by hepatocyte growth factor, hinders macrophage movement, suppresses progesterone and a luteinization inhibitor, and potentially contributes to embryo implantation and uterine lining changes in rats. In mature fat cells, it might act as a survival factor. A larger variant, Big STC, is linked to its potential role in pregnancy. STC1 is also recognized as a gene that responds to low oxygen levels, participating in the formation of blood vessels in such conditions. Interestingly, STC1 is being explored as a potential marker to detect tumor cells in blood and bone marrow across different cancer types.
Description
Human Stanniocalcin-1, a glycosylated protein, is produced in a 293 cell line. It consists of a single polypeptide chain with 240 amino acids, resulting in a molecular weight of 25.9 kDa. For identification purposes, it includes a C-Terminal Flag-tag consisting of 10 residues. The purification of Stanniocalcin is achieved using specialized chromatographic methods.
Physical Appearance
White, lyophilized (freeze-dried) powder.
Formulation
The product is filtered through a 0.4µm filter and then lyophilized. The lyophilized form is prepared in a 20mM Tris buffer with 50mM NaCl at pH 7.5, resulting in a concentration of 0.5mg/ml.
Solubility
To prepare a working solution, add deionized water to the lyophilized pellet until it reaches a concentration of approximately 0.5mg/ml and ensure complete dissolution. Please note that the product is not sterile. Before using it for cell culture, it's essential to filter it using an appropriate sterile filter.
Stability
Lyophilized STC-1 remains stable at room temperature for up to 3 weeks. However, for extended storage, it is recommended to store it in a desiccated condition below -18°C. Once reconstituted, Stanniocalcin should be stored at 4°C for a maximum of 2-7 days. For long-term storage, freezing below -18°C is advised. To preserve its stability during storage, it is recommended to add a carrier protein, either HSA or BSA, at a concentration of 0.1%. Avoid repeated freeze-thaw cycles to maintain product integrity.
Purity
The purity of the Stanniocalcin is determined to be greater than 95.0% through SDS-PAGE analysis.
Synonyms
Stanniocalcin-1, STC, STC-1.
Source
293 cell line (Human embryonic kidney).
Amino Acid Sequence
THEAEQNDSV SPRKSRVAAQ NSAEVVRCLN SALQVGCGAF ACLENSTCDT DGMYDICKSF LYSAAKFDTQ GKAFVKESLK CIANGVTSKV FLAIRRCSTF QRMIAEVQEE CYSKLNVCSI AKRNPEAITE VVQLPNHFSN RYYNRLVRSL LECDEDTVST IRDSLMEKIG PNMASLFHIL QTDHCAQTHP RADFNRRRTN EPQKLKVLLR NLRGEEDSPS HIKRTSHESA ASDYKDDDDK.
Product Science Overview
Discovery and Evolution
STC-1 was first discovered in bony fishes, where it functions to regulate calcium and phosphate levels. In mammals, including humans, STC-1 has evolved to perform a variety of roles beyond its initial function. It is involved in several physiological processes, including the inhibition of parathyroid hormone (PTH) and calcitonin, which are critical for bone metabolism .
Structure and Properties
STC-1 is a homodimeric protein, meaning it consists of two identical subunits. The human recombinant form of STC-1 is typically produced in HEK293 cells and has a molecular weight ranging from 28 to 36 kDa . The protein is often tagged with a C-terminal His-tag to facilitate purification and detection .
Biological Functions
In humans, STC-1 has been found to have multiple functions:
- Calcium and Phosphate Homeostasis: Similar to its role in fishes, STC-1 helps regulate calcium and phosphate levels in the body .
- Cancer Biology: STC-1 has been implicated in cancer progression. It inhibits the breakdown of PAPP-A, protects cancer cells from apoptosis, and reduces tumor size in liver cancers .
- Bone Metabolism: STC-1 promotes osteoblast differentiation and inhibits longitudinal bone growth directly at the growth plate .
- Neuroprotection: It serves as a biomarker for brain and lung cancer progression .
Applications in Research
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