SRP19 Human

Signal Recognition Particle 19kDa Human Recombinant
Cat. No.
BT26645
Source
Escherichia Coli.
Synonyms
Signal recognition particle 19kDa protein, SRP19.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SRP19 Human Recombinant fused with a 23 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 167 amino acids (1-144 a.a.) and having a molecular mass of 18.5kDa. The SRP19 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Signal recognition particle 19kDa protein (SRP19) is a protein belonging to the SRP19 family. It plays a crucial role in protein targeting by binding directly to 7S RNA. This interaction facilitates the binding of the 54 kDa subunit of the signal recognition particle (SRP).
Description
SRP19 Human Recombinant is a genetically engineered protein produced in E. coli. It consists of a single, non-glycosylated polypeptide chain containing 167 amino acids (with amino acids 1-144 representing the SRP19 sequence) and a 23 amino acid His tag at the N-terminus. This recombinant protein has a molecular weight of 18.5 kDa and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized through filtration.
Formulation
The SRP19 solution is provided at a concentration of 0.25 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.2 M NaCl, 50% glycerol, and 2 mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the SRP19 solution should be kept refrigerated at 4°C. For long-term storage, it is recommended to store the solution at -20°C. To ensure optimal stability during long-term storage, consider adding a carrier protein such as HSA or BSA at a concentration of 0.1%. Repeated freezing and thawing of the solution should be avoided.
Purity
The purity of the SRP19 Human Recombinant is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
Signal recognition particle 19kDa protein, SRP19.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMACAAAR SPADQDRFIC IYPAYLNNKK TIAEGRRIPI SKAVENPTAT EIQDVCSAVG LNVFLEKNKM YSREWNRDVQ YRGRVRVQLK QEDGSLCLVQ FPSRKSVMLY AAEMIPKLKT RTQKTGGADQ SLQQGEGSKK GKGKKKK.

Product Science Overview

Introduction

The Signal Recognition Particle (SRP) is a ribonucleoprotein complex that plays a crucial role in targeting nascent secretory and membrane proteins to the endoplasmic reticulum (ER) in eukaryotic cells and to the plasma membrane in prokaryotic cells . The SRP complex is composed of a 300-nucleotide RNA molecule (SRP RNA) and six polypeptides: SRP9, SRP14, SRP19, SRP54, SRP68, and SRP72 .

Structure and Function

SRP19 is one of the essential protein components of the SRP complex. It is a 19 kDa protein that binds directly to the 7SL RNA, a critical component of the SRP RNA . SRP19 is responsible for mediating the binding of SRP54 to the SRP complex, which is essential for the SRP’s function in protein targeting .

Nuclear Import and Assembly

Interestingly, SRP19 is imported into the nucleus by members of the importin β superfamily of transport receptors, specifically importin 8 and transportin . This nuclear import is crucial for the assembly of the SRP complex, as SRP RNA is synthesized in the nucleus and the SRP functions in the cytoplasm . The assembly process involves SRP19 binding to the SRP RNA in the nucleus before the complex is exported to the cytoplasm .

Recombinant SRP19

Recombinant human SRP19 is produced using techniques that involve expressing the protein in a host organism, such as Escherichia coli, and purifying it for research and therapeutic purposes . This recombinant protein retains the functional properties of the native SRP19, making it valuable for studying the SRP complex’s structure and function.

Applications and Research

Research on SRP19 has provided significant insights into the mechanisms of protein targeting and the role of SRP in cellular processes. Understanding SRP19’s function and its interactions within the SRP complex can lead to advancements in biotechnology and medicine, particularly in the development of therapeutic proteins and the study of diseases related to protein targeting and transport.

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