SRP14 Human
Description
Product Specs
Product Science Overview
Signal Recognition Particle 14kDa (SRP14) is a crucial component of the signal recognition particle (SRP) complex, which plays a vital role in the targeting and translocation of proteins to the endoplasmic reticulum (ER). This process is essential for the proper functioning of cellular activities, as it ensures that proteins are correctly localized within the cell.
SRP14 is a ribonucleoprotein complex that forms a heterodimer with SRP9. This heterodimer binds to the 5’ and 3’ terminal sequences of SRP RNA, forming the “Alu domain” of the SRP . The binding of SRP9/14 to SRP RNA is crucial for the transcription, maturation, nucleolus localization, and transport of SRP RNA .
The SRP complex, including SRP14, mediates the targeting of nascent polypeptide chains to the ER membrane. This targeting is achieved through the recognition of signal sequences on the nascent polypeptides by the SRP, which then directs the ribosome-nascent chain complex to the ER membrane .
Recombinant human SRP14 is a version of the protein that has been produced using recombinant DNA technology. This involves inserting the gene encoding SRP14 into a suitable expression system, such as E. coli, to produce the protein in large quantities . The recombinant SRP14 protein is often tagged with a His-tag at the N-terminus to facilitate purification and detection .
The recombinant human SRP14 protein is typically used in research to study the function and structure of the SRP complex, as well as its role in protein targeting and translocation. It is also used in various biochemical assays and structural studies to understand the interactions between SRP14 and other components of the SRP complex .
Recent studies have highlighted the potential clinical significance of SRP14. For instance, high expression levels of SRP14 have been associated with poor prognosis in patients with acute myeloid leukemia (AML) . Genome-wide co-expression analysis suggests that SRP14 may play a role in AML by participating in the regulation of various biological processes and signaling pathways, such as cell cycle, cell adhesion, and NF-κB signaling .
