SRGN Human, HEK
Serglycin Human Recombinant, HEK
Cat. No.
BT22268
Source
HEK 293.
Synonyms
Serglycin, PRG, PRG1, PPG, Proteoglycan 1 secretory granule, Hematopoetic proteoglycan core protein, Platelet proteoglycan core protein, proteoglycan protein core for mast cell secretory granule.
Appearance
Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Serglycin Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (Tyr28-Leu158) containing a total of 137 amino acids, having a calculated molecular mass of 15.5kDa and fused to a 6 aa His tag at C-Terminus.
Product Specs
Introduction
SRGN, a hematopoietic cell granule proteoglycan, plays a crucial role in neutralizing hydrolytic enzymes. This proteoglycan, found within the secretory granules of various hematopoietic cells, possesses a protease-resistant peptide core. Importantly, SRGN is linked to the macromolecular complex of granzymes and perforin, which mediates granule-mediated apoptosis.
Description
Recombinant Human Serglycin, produced in HEK cells, is a single, glycosylated polypeptide chain. This protein comprises 137 amino acids (Tyr28-Leu158), resulting in a calculated molecular mass of 15.5 kDa. It also includes a 6 aa His tag fused at the C-terminus.
Physical Appearance
Clear, colorless solution.
Formulation
SRGN is supplied as a 0.4 µm filtered solution in phosphate-buffered saline (PBS) containing 20% (w/v) glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 95.0% by SDS-PAGE analysis.
Synonyms
Serglycin, PRG, PRG1, PPG, Proteoglycan 1 secretory granule, Hematopoetic proteoglycan core protein, Platelet proteoglycan core protein, proteoglycan protein core for mast cell secretory granule.
Source
HEK 293.
Amino Acid Sequence
YPTRRARYQW VRCNPDSNSA NCLEEKGPMF ELLPGESNKI PRLRTDLFPK TRIQDLNRIF PLSEDYSGSG FGSGSGSGSG SGSGFLTEME QDYQLVDESD AFHDNLRSLD RNLPSDSQDL GQHGLEEDFM L HHHHHH.
Product Science Overview
Structure and Expression
Recombinant human serglycin is typically produced in HEK 293 cells (Human Embryonic Kidney cells). The recombinant form is a glycosylated polypeptide chain, often tagged with a His tag at the C-terminus for purification purposes . The protein consists of 137 amino acids, with a calculated molecular mass of approximately 15.5 kDa .
Functions
Serglycin has several important biological functions:
- Formation of Secretory Granules: It is essential for the formation of secretory granules in mast cells, where it mediates the storage of various compounds .
- Storage of Proteases: It is required for the storage of certain proteases in connective tissue and mucosal mast cells, as well as for the storage of granzyme B in T-lymphocytes .
- Localization of Neutrophil Elastase: Serglycin plays a role in localizing neutrophil elastase in azurophil granules of neutrophils .
- Processing of MMP2: It mediates the processing of matrix metalloproteinase 2 (MMP2) .
- Cytotoxic Cell Granule-Mediated Apoptosis: Serglycin forms a complex with granzyme B, which is delivered to target cells by perforin to induce apoptosis .
- Regulation of Secretion: It regulates the secretion of tumor necrosis factor-alpha (TNF-alpha) and may also regulate protease secretion .
- Inhibition of Bone Mineralization: Serglycin inhibits bone mineralization .
Clinical and Research Applications
Recombinant human serglycin is used in various research applications, including studies on inflammation, malignancy, and apoptosis. Its role in storing and regulating the release of inflammatory mediators makes it a valuable tool for understanding inflammatory responses and developing therapeutic interventions .
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