SRGN Human
Serglycin Human Recombinant
Cat. No.
BT22168
Source
E.coli.
Synonyms
Serglycin, PRG, PRG1, PPG, Proteoglycan 1 secretory granule, Hematopoetic proteoglycan core protein, Platelet proteoglycan core protein, proteoglycan protein core for mast cell secretory granule.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
SRGN Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 156 amino acids (28-158) and having a molecular mass of 17.4 kDa (Molecular weight on SDS-PAGE will appear higher).
SRGN is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
SRGN is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
SRGN, a hematopoietic cell granule proteoglycan, plays a crucial role in the function of secretory granules found in various hematopoietic cells. This proteoglycan features a protease-resistant peptide core, which is essential for neutralizing harmful hydrolytic enzymes. Notably, SRGN is associated with the macromolecular complex of granzymes and perforin, acting as a key mediator in granule-mediated apoptosis.
Description
Recombinant SRGN Human, produced in E.coli, is a single, non-glycosylated polypeptide chain comprising 156 amino acids (28-158) with a molecular mass of 17.4 kDa. Note that the molecular weight on SDS-PAGE might appear higher. This protein is fused to a 25 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The SRGN solution is provided at a concentration of 1mg/ml and is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 2mM DTT, 0.15M NaCl, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the solution can be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freezing and thawing of the solution should be avoided.
Purity
The purity of the SRGN protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
Serglycin, PRG, PRG1, PPG, Proteoglycan 1 secretory granule, Hematopoetic proteoglycan core protein, Platelet proteoglycan core protein, proteoglycan protein core for mast cell secretory granule.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMYPTRR ARYQWVRCNP DSNSANCLEE KGPMFELLPG ESNKIPRLRT DLFPKTRIQD LNRIFPLSED YSGSGFGSGS GSGSGSGSGF LTEMEQDYQL VDESDAFHDN LRSLDRNLPS DSQDLGQHGL EEDFML.
Product Science Overview
Definition and Classification
Biological Properties
Expression Patterns and Tissue Distribution
Biological Functions
Serglycin has several key functions:
- Storage of Proteases: It is essential for the storage of proteases in connective tissue and mucosal mast cells, as well as granzyme B in T-lymphocytes .
- Granule Homeostasis: It maintains the homeostasis of secretory granules in mast cells, affecting their morphology and staining properties .
- Localization of Enzymes: Serglycin localizes neutrophil elastase in the azurophil granules of neutrophils .
Roles in Immune Responses and Pathogen Recognition
Modes of Action and Mechanisms with Other Molecules and Cells
Binding Partners and Downstream Signaling Cascades
Regulatory Mechanisms
The expression and function of serglycin are regulated by various factors, including local growth factors such as stem cell factor (SCF) that influence mast cell development and function .
Serglycin’s multifaceted roles in immune responses, enzyme storage, and granule homeostasis make it a critical component in various physiological processes.
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