SPA-Cys Long
Staphylococcal Protein-A Cys Long Form Recombinant
Cat. No.
BT10491
Source
Escherichia Coli.
Synonyms
Immunoglobulin G-binding protein A, IgG-binding protein A, Staphylococcal protein A, SPA.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
SPA-Cys long Recombinant produced in E.Coli is a single non-glycosylated polypeptide chain with a Cys on C-terminus. SPA-Cys is comprised of 5 IgG-binding domains E-D-A-B-C aligned in series containing 423 amino acids and having a molecular mass of 46.7kDa containing little or no carbohydrate.
Product Specs
Introduction
Protein A, a cell wall component found in certain Staphylococcus aureus strains, plays a crucial role in antibody purification. Our recombinant Protein A, genetically engineered with five IgG-binding regions, offers a reliable alternative to native Protein A for purifying polyclonal and monoclonal IgG antibodies. This versatile protein exhibits binding affinity to specific IgG subclasses from various species, including human IgG1, IgG2, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2c; and total IgG from rabbit, pig, dog, cat, and guinea pig.
Description
Produced in E. Coli, our SPA-Cys Long Recombinant is a non-glycosylated polypeptide chain with a C-terminal Cys residue. This protein consists of five tandemly arranged IgG-binding domains (E-D-A-B-C), encompassing 423 amino acids. With a molecular mass of 46.7kDa, SPA-Cys Long contains minimal to no carbohydrates.
Physical Appearance
White, lyophilized powder, sterile filtered.
Formulation
The SPA protein is lyophilized without any additional additives.
Solubility
To reconstitute the lyophilized SPA, it is advised to dissolve it in sterile 18MΩ-cm H2O at a concentration of at least 0.1mg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized SPA remains stable for up to 3 weeks at room temperature but should ideally be stored in desiccated conditions below -18°C. After reconstitution, store SPA at 4°C for no more than 2-7 days. For extended storage, freezing below -18°C is recommended, preferably with the addition of a carrier protein like HSA or BSA (0.1%). Avoid repeated freeze-thaw cycles.
Purity
Our SPA-Cys Long Recombinant boasts a purity exceeding 97.0%, as determined by SDS-PAGE analysis.
Synonyms
Immunoglobulin G-binding protein A, IgG-binding protein A, Staphylococcal protein A, SPA.
Source
Escherichia Coli.
Amino Acid Sequence
AQHDEAQQNA FYQVLNMPNL NADQRNGFIQ SLKDDPSQSA NVLGEAQKLN DSQAPKADAQ QNNFNKDQQS AFYEILNMPN LNEAQRNGFI QSLKDDPSQS TNVLGEAKKL NESQAPKADN NFNKEQQNAF YEILNMPNLN EEQRNGFIQS LKDDPSQSAN LLSEAKKLNE SQAPKADNKF NKEQQNAFYE ILHLPNLNEE QRNGFIQSLK DDPSQSANLL AEAKKLNDAQ APKADNKFNK EQQNAFYEIL HLPNLTEEQR NGFIQSLKDD PSVSKEILAE AKKLNDAQAP KEEDNKKPGK EDGNKPGKED GNKPGKEDNK KPGKEDGNKP GKEDNNKPGK EDGNKPGKED NNKPGKEDGN KPGKEDGNKP GKEDGNGVHV VKPGDTVNDI AKANGTTADK IAADNKLADK NMIKPGQELV VDC
Product Science Overview
Structure and Function
The recombinant form of Staphylococcal Protein A, specifically the Cys Long Form, is a genetically engineered version of the native protein. This recombinant protein retains the essential properties of the native SPA, including its ability to bind to IgG antibodies. The Cys Long Form is characterized by the presence of five IgG-binding regions, which enhances its binding capacity and makes it particularly useful for antibody purification processes .
Applications
Recombinant Staphylococcal Protein A Cys Long Form is widely used in various applications, including:
- Antibody Purification: Due to its high affinity for IgG antibodies, SPA is commonly used in the purification of polyclonal and monoclonal antibodies. This is crucial for research and therapeutic applications where high-purity antibodies are required.
- Immunoprecipitation: SPA can be used to isolate and concentrate specific antigens from complex mixtures, facilitating the study of protein-protein interactions and other biochemical processes.
- Diagnostic Assays: The binding properties of SPA make it a valuable component in diagnostic assays, where it can be used to detect the presence of specific antibodies in a sample.
Production and Purification
The production of recombinant SPA involves the expression of the protein in a suitable host organism, such as Escherichia coli. The protein is then purified through a series of chromatographic techniques to ensure high purity and functionality. The Cys Long Form is often lyophilized to enhance its stability and shelf life .
Advantages of Recombinant SPA
- Consistency: Recombinant production ensures a consistent supply of SPA with uniform properties, which is essential for reproducible results in research and diagnostic applications.
- Safety: Recombinant SPA is free from contaminants that may be present in native preparations, reducing the risk of introducing unwanted variables into experiments.
- Versatility: The Cys Long Form of SPA can be easily modified to include additional functional groups or tags, expanding its utility in various experimental setups.
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