SPA-Cys His
Description
Product Specs
Product Science Overview
Recombinant Protein-A is a genetically engineered version of the native protein. It retains the functional properties of the native protein, including the ability to bind IgG. The recombinant form is often preferred in research and industrial applications due to its consistency and purity.
The recombinant Staphylococcal Protein-A Cys, His Tag, is designed to include a cysteine residue and a polyhistidine tag (His Tag). The cysteine residue allows for site-specific conjugation, while the His Tag facilitates purification through immobilized metal affinity chromatography (IMAC). This recombinant protein typically consists of five IgG-binding domains (E, D, A, B, C) aligned in series, containing 307 amino acids and having a molecular mass of approximately 34.8 kDa .
Recombinant Protein-A is widely used in the purification of polyclonal and monoclonal IgG antibodies. Its ability to bind the Fc region of IgG makes it an essential tool in affinity chromatography, where it helps isolate antibodies from complex mixtures. Additionally, it is used in immunoprecipitation and immunoassays to detect and quantify antibodies.
- Consistency: Recombinant production ensures batch-to-batch consistency, which is crucial for reproducible results in research and industrial applications.
- Purity: The recombinant form is free from contaminants that may be present in native preparations.
- Versatility: The addition of tags such as the His Tag allows for easy purification and conjugation, enhancing the protein’s utility in various applications.
