SOST Human, HEK
HEK293 Cells.
Sclerostin, SOST, CDD, VBCH.
Greater than 95.0% as determined by SDS-PAGE.
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Description
SOST Human Recombinant produced in HEK293 cells is a single, glycosylated polypeptide chain (a.a 24-213) containing 196 amino acids including a 6 a.a C-terminal His tag. The total molecular mass is 22.4kDa (calculated).
Product Specs
Sclerostin (SOST), a secreted glycoprotein belonging to the DAN family, acts as a regulator of bone formation. Characterized by its C-terminal cysteine knot-like (CTCK) domain, SOST shares similarities with bone morphogenetic protein (BMP) antagonists. Its primary role involves inhibiting bone growth. SOST exhibits widespread expression at low levels, with heightened concentrations found in bone, cartilage, kidney, liver, bone marrow, and mature osteoblasts. Genetic defects in the SOST gene are associated with sclerosteosis and bone dysplasia.
Recombinant Human SOST, produced in HEK293 cells, is a single, glycosylated polypeptide chain. It encompasses amino acids 24-213, totaling 196 amino acids, and includes a 6 amino acid C-terminal His tag. The calculated molecular mass is 22.4 kDa.
SOST is filtered through a 0.4 μm filter and lyophilized from a solution of 0.5 mg/ml in phosphate-buffered saline (PBS) containing 5% (w/v) trehalose at a pH of 7.4.
To prepare a working stock solution, add deionized water to the lyophilized pellet to achieve a concentration of approximately 0.5 mg/ml. Ensure complete dissolution. This product is not sterile. Prior to cell culture use, filter the solution through an appropriate sterile filter.
Store the lyophilized protein at -20°C. After reconstitution, aliquot the product to minimize repeated freeze-thaw cycles. Reconstituted protein remains stable at 4°C for a limited period and shows no significant changes within one week at this temperature.
Purity is determined to be greater than 95.0% by SDS-PAGE analysis.
Sclerostin, SOST, CDD, VBCH.
HEK293 Cells.
QGWQAFKNDA TEIIPELGEY PEPPPELENN KTMNRAENGG RPPHHPFETK DVSEYSCREL HFTRYVTDGP CRSAKPVTEL VCSGQCGPAR LLPNAIGRGK WWRPSGPDFR CIPDRYRAQR VQLLCPGGEA PRARKVRLVA SCKCKRLTRF HNQSELKDFG TEAARPQKGR KPRPRARSAK ANQAELENAY HHHHHH.
Product Science Overview
Sclerostin is a secreted glycoprotein encoded by the SOST gene. It plays a crucial role in bone metabolism by acting as an antagonist to the Wnt signaling pathway, which is essential for bone formation and remodeling . The recombinant form of sclerostin, expressed in Human Embryonic Kidney (HEK) 293 cells, is used extensively in research and therapeutic applications.
Sclerostin is a monomeric protein with a C-terminal cysteine knot-like (CTCK) domain, sharing sequence similarity with the DAN family of bone morphogenetic protein (BMP) antagonists . It is primarily produced by osteocytes, the most abundant cells in mature bone . Sclerostin inhibits the Wnt/β-catenin signaling pathway by binding to low-density lipoprotein receptor-related protein (LRP) 5/6, thereby suppressing osteoblast differentiation and function .
The recombinant form of sclerostin is expressed in HEK 293 cells, a widely used cell line derived from human embryonic kidney cells. This expression system is preferred due to its ability to produce high yields of properly folded and post-translationally modified proteins . The recombinant sclerostin is typically tagged with a polyhistidine (His) tag to facilitate purification and detection .
Sclerostin has garnered significant attention due to its role in bone diseases such as osteoporosis. Inhibition of sclerostin has been shown to increase bone formation and density, making it a target for therapeutic interventions . Romosozumab, a monoclonal antibody against sclerostin, has been developed to treat osteoporosis by promoting bone formation and reducing fracture risk .
