SHH Mouse

Sonic Hedgehog Mouse Recombinant
Cat. No.
BT24517
Source
Escherichia Coli.
Synonyms
SHH, HHG-1, HHG1, Sonic hedgehog protein.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Sonic Hedgehog Recombinant Mouse produced in E.Coli is a single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 19.8 kDa. The Mouse Sonic Hedgehog is 99% homologous to the human gene.
Cysteine at position 25 has been substituted with Ile.
The Sonic HedgeHog is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Recombinant Mouse Sonic Hedgehog is a member of a small family of secreted proteins crucial for development in both vertebrates and invertebrates. The three mammalian hedgehog genes (sonic, desert, and Indian) exhibit approximately 60% homology. Notably, Mouse Sonic Hedgehog shares 99% homology with its human counterpart. Sonic Hedgehog plays a vital role in guiding early embryonic development and has been identified as a primary inductive signal in the patterning of the ventral neural tube, the anterior-posterior limb axis, and ventral somites. Binding to the Patched receptor, Sonic Hedgehog, in conjunction with Smoothened, activates the transcription of target genes. In the absence of Sonic Hedgehog, the Patched receptor suppresses the inherent signaling activity of Smoothened. Furthermore, Sonic Hedgehog regulates the Gli oncogene. This intercellular signaling molecule is essential for various developmental patterning processes, including ventral cell fate induction in the neural tube and somites by signals from the notochord and anterior-posterior axis polarization in the developing limb bud. Sonic Hedgehog exhibits both floor plate- and motor neuron-inducing activities. Mutations in a long-range enhancer of Sonic Hedgehog, located within an intron of the limb region 1 gene, can lead to preaxial polydactyly.
Description
Recombinant Mouse Sonic Hedgehog, produced in E. coli, is an unglycosylated polypeptide chain composed of 176 amino acids, with a molecular weight of 19.8 kDa. It shares 99% homology with the human Sonic Hedgehog gene. The cysteine residue at position 25 has been replaced with isoleucine. Purification of Sonic Hedgehog is achieved through proprietary chromatographic methods.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 1 mg/mL solution in 10 mM sodium phosphate buffer (pH 7.5).
Solubility
Reconstitute the lyophilized Mouse Sonic Hedgehog in sterile 18 MΩ·cm H₂O to a concentration of at least 100 µg/mL. This solution can be further diluted in other aqueous solutions.
Stability
Lyophilized Mouse Sonic Hedgehog remains stable at room temperature for up to 3 weeks. However, for long-term storage, it should be kept desiccated below -18 °C. After reconstitution, store Sonic Hedgehog at 4 °C for 2-7 days. For extended storage, freeze aliquots below -18 °C. Avoid repeated freeze-thaw cycles.
Purity
Greater than 97.0% as determined by SDS-PAGE.
Biological Activity
The biological activity is determined by the dose-dependent induction of alkaline phosphatase production in C3H/10T1/2 (CCL-226) fibroblasts and is typically 0.48-0.72 µg/mL.
Synonyms
SHH, HHG-1, HHG1, Sonic hedgehog protein.
Source
Escherichia Coli.
Amino Acid Sequence
MIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG.

Product Science Overview

Introduction

Sonic Hedgehog (Shh) is a highly conserved protein that plays a crucial role in embryonic development. It is part of the Hedgehog signaling pathway, which is essential for the regulation of cell growth, differentiation, and tissue patterning. The name “Sonic Hedgehog” was inspired by the popular video game character, reflecting the protein’s significant impact on developmental biology.

Discovery and Nomenclature

The Hedgehog gene was first discovered in fruit flies (Drosophila melanogaster) in the 1980s. Researchers identified three homologs in vertebrates: Desert Hedgehog (Dhh), Indian Hedgehog (Ihh), and Sonic Hedgehog (Shh). Among these, Shh is the most extensively studied due to its critical role in various developmental processes.

Structure and Function

Sonic Hedgehog is a secreted protein that undergoes autocatalytic cleavage to produce an active N-terminal fragment. This fragment is responsible for the protein’s signaling activity. Shh binds to its receptor, Patched (Ptch), which in turn regulates the activity of another protein called Smoothened (Smo). This signaling cascade ultimately influences the expression of target genes involved in cell proliferation, differentiation, and tissue patterning.

Role in Development

Shh is expressed in several embryonic tissues that are vital for the patterning of the developing central nervous system, somite, and limb . It is also involved in the development of whiskers, hair, foregut, teeth, and bones . The protein’s gradient in tissues helps establish the spatial organization of cells, guiding the formation of various structures during embryogenesis.

Recombinant Mouse Sonic Hedgehog

Recombinant Mouse Sonic Hedgehog (Shh) is produced using E. coli expression systems. The recombinant protein typically includes the N-terminal signaling domain, which retains its biological activity. It is used in various research applications to study the Hedgehog signaling pathway and its role in development and disease.

Applications in Research

Recombinant Shh is utilized in studies involving:

  • Cell Differentiation: Investigating how Shh influences the differentiation of stem cells into specific cell types.
  • Tissue Engineering: Exploring the potential of Shh in regenerating damaged tissues and organs.
  • Cancer Research: Understanding the role of aberrant Shh signaling in the development and progression of cancers, such as basal cell carcinoma and medulloblastoma.
  • Drug Development: Screening for compounds that can modulate the Hedgehog signaling pathway, offering potential therapeutic strategies for various diseases.

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