DHH (C23II) Human

Desert Hedgehog (C23II) Human Recombinant
Cat. No.
BT23890
Source
Escherichia Coli.
Synonyms
HHG-3, Desert Hedgehog homolog, MGC35145, Desert hedgehog protein, DHH.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

DHH (C23II) Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 177 amino acids and having a molecular mass of 19.9kDa.
The DHH (C23II) is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Desert Hedgehog (DHH) is a member of the Hedgehog family, which encodes signaling molecules crucial for regulating morphogenesis. DHH protein undergoes autocatalytic cleavage, resulting in an N-terminal fragment containing the signaling activity and a C-terminal fragment involved in precursor processing. The C-terminal fragment facilitates the attachment of a cholesterol moiety to the N-terminal fragment, restricting it to the cell surface and preventing its free diffusion. DHH mutations are linked to partial gonadal dysgenesis (PGD) with minifascicular polyneuropathy. DHH is essential for male gonadal differentiation, perineurial development, and acts as a spermatocyte survival factor in the testes, playing a critical role in testes development.
Description
Recombinant human DHH (C23II) is produced in E. coli. It is a single, non-glycosylated polypeptide chain comprising 177 amino acids with a molecular weight of 19.9 kDa. The purification of DHH (C23II) is achieved using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The product is lyophilized from a 0.2 µm filtered solution concentrated in 1x PBS with a pH of 7.4.
Solubility
For reconstitution, it is recommended to dissolve the lyophilized DHH (C23II) in sterile 18 MΩ·cm H₂O at a concentration of at least 100 µg/ml. This solution can be further diluted with other aqueous solutions.
Stability
Lyophilized DHH (C23II) remains stable at room temperature for up to 3 weeks. However, it is recommended to store the lyophilized product desiccated at a temperature below -18°C. After reconstitution, DHH (C23II) can be stored at 4°C for 2-7 days. For prolonged storage, it is advisable to store the reconstituted solution at a temperature below -18°C with the addition of a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Purity
The purity of the product is greater than 95.0% as determined by: (a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis and (b) SDS-polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
Biological Activity
The biological activity of DHH (C23II) is assessed by its ability to stimulate alkaline phosphatase production in C3H/10T1/2 (CCL-226) cells. The expected half-maximal effective concentration (ED₅₀) for this effect is in the range of 15-45 µg/ml.
Synonyms
HHG-3, Desert Hedgehog homolog, MGC35145, Desert hedgehog protein, DHH.
Source
Escherichia Coli.
Amino Acid Sequence
IIGPGRGPVG RRRYARKQLV PLLYKQFVPG VPERTLGASG PAEGRVARGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH VHVSVKADNS LAVRAGG.

Product Science Overview

Introduction

Desert Hedgehog (DHH) is a member of the highly conserved Hedgehog family of proteins, which play crucial roles in various developmental processes. The Hedgehog family includes three proteins in mammals: Sonic Hedgehog (SHH), Indian Hedgehog (IHH), and Desert Hedgehog (DHH). These proteins are involved in cell signaling pathways that regulate embryonic development, tissue patterning, and stem cell maintenance.

Structure and Function

Desert Hedgehog (DHH) is a secreted protein that undergoes autocatalytic cleavage, resulting in two functional domains: the N-terminal signaling domain and the C-terminal autoprocessing domain. The N-terminal domain is responsible for the protein’s signaling activity, while the C-terminal domain facilitates the cleavage and attachment of cholesterol to the N-terminal domain, which is essential for its proper functioning .

The recombinant form of Desert Hedgehog (C23II) is produced in E. coli and consists of a single, non-glycosylated polypeptide chain containing 177 amino acids. This recombinant protein is biologically active and has been shown to induce alkaline phosphatase production in specific cell lines .

Biological Activity

Desert Hedgehog (C23II) plays a significant role in various biological processes, including:

  • Embryonic Development: DHH is crucial for the development of the gonads and peripheral nerves. It is involved in the differentiation of Sertoli cells in the testes and Schwann cells in the peripheral nervous system.
  • Tissue Patterning: DHH signaling is essential for the proper patterning of tissues during embryogenesis. It helps in the formation of various organs and structures by regulating cell proliferation, differentiation, and apoptosis.
  • Stem Cell Maintenance: DHH is involved in the maintenance of stem cell populations in certain tissues, ensuring the proper regeneration and repair of tissues throughout an organism’s life .
Applications

Recombinant Desert Hedgehog (C23II) is widely used in research to study its role in developmental biology and disease. Some of its applications include:

  • Cell Signaling Studies: Researchers use recombinant DHH to investigate the mechanisms of Hedgehog signaling pathways and their impact on cell behavior.
  • Developmental Biology: DHH is used to study the processes of embryonic development and tissue patterning, providing insights into congenital disorders and developmental abnormalities.
  • Stem Cell Research: DHH is utilized to explore its role in stem cell maintenance and differentiation, contributing to advancements in regenerative medicine and tissue engineering .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

DHH (C23II) Mouse
Desert Hedgehog (C23II) Mouse Recombinant
Cat. No.
BT23966
Source
Escherichia Coli.
DHH Human
Desert Hedgehog Human Recombinant
Cat. No.
BT24041
Source
Escherichia Coli.
SHH Mouse
Sonic Hedgehog Mouse Recombinant
Cat. No.
BT24517
Source
Escherichia Coli.
SHH Mouse, His
Sonic HedgeHog Mouse Recombinant, His Tag
Cat. No.
BT24727
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.