SNTA1 Human

Syntrophin, Alpha 1 Human Recombinant
Cat. No.
BT18024
Source
E.coli.
Synonyms
Alpha-1-syntrophin, 59 kDa dystrophin-associated protein A1 acidic component 1, Pro-TGF-alpha cytoplasmic domain-interacting protein 1, TACIP1, Syntrophin-1, SNTA1, SNT1, LQT12, dJ1187J4.5.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 80% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SNTA1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 528 amino acids (1-505 a.a.) and having a molecular mass of 56.3kDa.
SNTA1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
SNTA1, a member of the syntrophin gene family, is a peripheral membrane protein that associates with dystrophin and dystrophin-related proteins. Dystrophin, a large, rod-like cytoskeletal protein found on the inner surface of muscle fibers, is absent in Duchenne Muscular Dystrophy patients and present in reduced amounts in Becker Muscular Dystrophy patients. Syntrophins function as cytoplasmic peripheral membrane scaffold proteins and are integral components of the dystrophin-associated protein complex. The N-terminal PDZ domain of SNTA1 interacts with the C-terminus of the pore-forming alpha subunit (SCN5A) of the cardiac sodium channel Nav1.5. Furthermore, SNTA1 links cardiac sodium channels to the nitric oxide synthase-PMCA4b (plasma membrane Ca-ATPase subtype 4b) complex in cardiomyocytes. The SNTA1 gene has been identified as a predisposition locus for Long-QT syndrome (LQT), an inherited disorder characterized by sudden cardiac death from arrhythmia, and sudden infant death syndrome (SIDS). SNTA1 also interacts with dystrophin and dystrophin-related proteins at the neuromuscular junction, influencing intracellular calcium ion levels in muscle tissue.
Description
Recombinant human SNTA1, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 528 amino acids (1-505 a.a.). This protein has a molecular weight of 56.3 kDa and includes a 23 amino acid His-tag at the N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The SNTA1 protein solution is provided at a concentration of 1 mg/ml and contains the following components: 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1 mM DTT, and 0.15 M NaCl.
Stability
For optimal storage, the entire vial should be stored at 4°C and used within 2-4 weeks. For extended storage periods, it is recommended to freeze the protein at -20°C. The addition of a carrier protein, such as 0.1% HSA or BSA, is advisable for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of the SNTA1 protein is determined to be greater than 80% based on SDS-PAGE analysis.
Synonyms
Alpha-1-syntrophin, 59 kDa dystrophin-associated protein A1 acidic component 1, Pro-TGF-alpha cytoplasmic domain-interacting protein 1, TACIP1, Syntrophin-1, SNTA1, SNT1, LQT12, dJ1187J4.5.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMASGRRA PRTGLLELRA GAGSGAGGER WQRVLLSLAE DVLTVSPADG DPGPEPGAPR EQEPAQLNGA AEPGAGPPQL PEALLLQRRR VTVRKADAGG LGISIKGGRE NKMPILISKI FKGLAADQTE ALFVGDAILS VNGEDLSSAT HDEAVQVLKK TGKEVVLEVK YMKDVSPYFK NSTGGTSVGW DSPPASPLQR QPSSPGPTPR NFSEAKHMSL KMAYVSKRCT PNDPEPRYLE ICSADGQDTL FLRAKDEASA RSWATAIQAQ VNTLTPRVKD ELQALLAATS TAGSQDIKQI GWLTEQLPSG GTAPTLALLT EKELLLYLSL PETREALSRP ARTAPLIATR LVHSGPSKGS VPYDAELSFA LRTGTRHGVD THLFSVESPQ ELAAWTRQLV DGCHRAAEGV QEVSTACTWN GRPCSLSVHI DKGFTLWAAE PGAARAVLLR QPFEKLQMSS DDGASLLFLD FGGAEGEIQL DLHSCPKTIV FIIHSFLSAK VTRLGLLA.

Product Science Overview

Introduction

Syntrophin, Alpha 1 (Human Recombinant), also known as alpha-1-syntrophin, is a 58 kDa peripheral cytoplasmic membrane adaptor protein. It is a member of the syntrophin family and plays a crucial role in various cellular processes, including signal transduction and cytoskeletal dynamics.

Historical Background

Syntrophins were first identified in the postsynaptic membranes of the Torpedo electric organ. Alpha-1-syntrophin was the first isoform of the syntrophin family to be discovered and has since been cloned and characterized . It is encoded by the SNTA gene in humans and is 505 amino acids long .

Structure

Alpha-1-syntrophin has a unique domain organization that includes two PH-domains (PH1 and PH2), a PDZ domain, and a Syntrophin Unique (SU) domain . The PH1 domain is split by the PDZ domain, which is a unique feature of alpha-1-syntrophin . The PH2 domain and the SU domain are responsible for interactions between the dystrophin glycoprotein complex (DGC) and alpha-1-syntrophin .

Expression and Function

Alpha-1-syntrophin is expressed in various mammalian tissues, including skeletal muscles, heart, brain, stomach, and breasts . It forms part of the DGC in muscle cells and is concentrated at the neuromuscular junction in the brain . The protein plays a role in signal transduction mechanisms and cytoskeletal dynamics, contributing to the stability and function of muscle cells .

Recombinant Human Syntrophin Alpha 1

Recombinant Human Syntrophin Alpha 1 is produced using recombinant DNA technology. It is typically expressed in E. coli and purified for research and therapeutic purposes . The recombinant protein includes a N-terminal His-tag and corresponds to the amino acids 1-505 of Human Syntrophin Alpha 1 . It is used in various applications, including SDS-PAGE assays and other biochemical studies .

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