SNRPB Human
Greater than 95% as determined by SDS-PAGE.
Description
SNRPB Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 25.4 kDa which migrates at 30kDa on SDS-PAGE. SNRPB is expressed with a -6x His tag and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Small Nuclear Ribonucleoprotein Polypeptides B and B1 (SNRPB) are essential components of the spliceosome, a complex responsible for pre-mRNA splicing in eukaryotic cells. These polypeptides are encoded by the SNRPB gene and are found in U1, U2, U4/U6, and U5 small nuclear ribonucleoprotein particles (snRNPs) . The spliceosome is crucial for the removal of introns from pre-mRNA, a process necessary for the maturation of messenger RNA (mRNA) and subsequent protein synthesis.
SNRPB plays a pivotal role in the splicing of pre-mRNA by forming the core of the spliceosomal snRNPs . These snRNPs are involved in the recognition and excision of introns, ensuring the accurate processing of pre-mRNA into mature mRNA. The proper functioning of SNRPB is vital for gene expression and regulation, as errors in splicing can lead to various genetic disorders and diseases .
The recombinant production of SNRPB involves the cloning of the SNRPB gene into an appropriate expression vector, followed by the transformation of a suitable host cell, such as Escherichia coli or yeast . The host cells are then cultured under optimal conditions to express the recombinant protein. After expression, the protein is purified using techniques such as affinity chromatography, ion exchange chromatography, and gel filtration .
SNRPB interacts with other components of the spliceosome, including U1, U2, U4/U6, and U5 snRNPs, to facilitate the splicing process . It binds to specific RNA sequences and proteins, forming a complex that catalyzes the removal of introns from pre-mRNA . Additionally, SNRPB is involved in the assembly and stability of the spliceosome, ensuring the accurate and efficient splicing of pre-mRNA .
Mutations or dysregulation of the SNRPB gene can lead to various genetic disorders, including cerebrocostomandibular syndrome and rare diseases associated with Pierre Robin syndrome . Furthermore, autoantibodies against SNRPB are frequently found in patients with systemic lupus erythematosus, indicating its role in autoimmune diseases .
