SNRPB2 Human

SNRPB is one of several nuclear proteins found in common among U1, U2, U4/U6, and U5 snRNPs . These snRNPs are the building blocks of the spliceosome, a complex responsible for removing introns from pre-mRNA . The protein encoded by the SNRPB gene plays a significant role in the splicing process and may also contribute to the structure of snRNPs .

The SNRPB gene is located on chromosome 20 and encodes two isoforms, B and B’, through alternative splicing . These isoforms are involved in the formation of the spliceosome and are recognized by autoantibodies in patients with systemic lupus erythematosus .

Mutations or dysregulation of the SNRPB gene have been associated with various diseases, including cerebrocostomandibular syndrome and rare diseases with Pierre Robin syndrome . The protein’s involvement in pre-mRNA splicing makes it a critical target for understanding these conditions.

Recombinant SNRPB is produced using expression systems such as wheat germ . This recombinant protein is used in various research applications, including Western blotting (WB), enzyme-linked immunosorbent assay (ELISA), and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) . The recombinant form allows researchers to study the protein’s function and interactions in a controlled environment.

SNRPB is a subject of extensive research due to its fundamental role in RNA processing. Studies have shown that it is a component of both the pre-catalytic spliceosome B complex and the activated spliceosome C complexes . Additionally, it is involved in the splicing of U12-type introns in pre-mRNAs .