SNCG Mouse

Gamma-synuclein is a presynaptic protein, meaning it is located at the synapse, the junction between two nerve cells where communication occurs . The protein is involved in modulating synaptic function and plasticity, which are crucial for learning and memory. The exact mechanisms by which gamma-synuclein contributes to neurodegenerative diseases are still under investigation, but it is known to be a major component of the protein aggregates found in the brains of individuals with PD .

Recombinant gamma-synuclein is produced using genetic engineering techniques. A DNA sequence encoding the mouse gamma-synuclein protein is inserted into a bacterial host, such as Escherichia coli (E. coli), which then expresses the protein. The recombinant protein is subsequently purified to ensure high purity and quality .

Recombinant gamma-synuclein is used in various research applications, including:

• Aggregation Studies: Investigating how gamma-synuclein aggregates form and contribute to neurodegenerative diseases.
• Seeding Experiments: Studying the mechanisms by which gamma-synuclein aggregates propagate in the brain.
• Molecular Standards: Serving as a standard for various biochemical assays .

The recombinant gamma-synuclein is typically provided as a white lyophilized powder and should be stored at -20°C to maintain its stability. It is often dissolved in a buffer solution, such as 20mM Tris, 100mM NaCl, pH 7.4, to ensure a highly monomeric starting material .

Research on gamma-synuclein continues to provide insights into its role in neurodegenerative diseases. Understanding the protein’s structure, function, and aggregation properties could lead to the development of new therapeutic strategies for conditions like Parkinson’s Disease and other synucleinopathies .