SNCA Delta-NAC Human
Description
A-Synuclein Delta-NAC Human Recombinant which is a deletion mutant of the a-synuclein that lacks the NAC region (amino acid 61-95), produced in E.Coli is a single, non-glycosylated polypeptide chain of 111 amino acids having a molecular mass of 11.9kDa (molecular size on SDS-PAGE will appear higher), with 6 amino acids added as a linker. The Recombinant Human a-Synuclein Delta-NAC is purified by proprietary chromatographic techniques.
Product Specs
MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK GTEIWMKKDQ LGKNEEGAPQ EGILEDMPVD PDNEAYEMPS EEGYQDYEPE A.
Product Science Overview
Alpha-Synuclein (α-Synuclein) is a presynaptic protein predominantly found in neural tissue. It is a major component of Lewy bodies, which are protein aggregates that are a hallmark of Parkinson’s Disease (PD) and other neurodegenerative disorders . The protein is composed of 140 amino acids and is known for its role in synaptic vesicle regulation and neurotransmitter release .
Delta-NAC Variant: The Delta-NAC variant of alpha-synuclein is a deletion mutant that lacks the Non-Amyloid Component (NAC) region, specifically amino acids 61-95 . This region is crucial for the aggregation properties of alpha-synuclein, and its absence in the Delta-NAC variant makes it a valuable tool for studying the mechanisms of protein aggregation and its implications in neurodegenerative diseases .
Recombinant Production: The human recombinant Alpha Synuclein Delta-NAC is produced in Escherichia coli (E. coli) and purified to ensure high purity and consistency . The recombinant protein is typically expressed as a single, non-glycosylated polypeptide chain of 111 amino acids, with an additional 6 amino acids added as a linker . The molecular mass of this recombinant protein is approximately 11.9 kDa .
Applications: The recombinant Alpha Synuclein Delta-NAC is used in various research applications, including:
- Aggregation Studies: Understanding the aggregation properties of alpha-synuclein and its role in neurodegenerative diseases .
- Seeding Experiments: Investigating the seeding and propagation of alpha-synuclein aggregates .
- Molecular Standards: Serving as a standard in biochemical assays and experiments .
Storage and Stability: The recombinant protein is typically stored in a buffer containing 20mM Tris-HCl (pH 7.5) and 100mM NaCl . For long-term storage, it is recommended to keep the protein at -20°C and avoid multiple freeze-thaw cycles to maintain its stability .
Conclusion: The human recombinant Alpha Synuclein Delta-NAC is a crucial tool for researchers studying the pathogenesis of Parkinson’s Disease and related neurodegenerative disorders. By providing a highly purified and consistent protein, it enables detailed investigations into the molecular mechanisms underlying protein aggregation and its impact on neuronal function.
